WormMine: RNAi WBRNAi00087466

• WormBase ID: WBRNAi00087466
• Phenotype Remark: Each worm laid about 50 to 100 eggs during its reproductive life span, while uninjected hermaphrodite that has not mated normally lays about 300 eggs. About 20% of the eggs developed to adult stage (F1 progeny), and 80% died during early embryogenesis. The number of eggs laid by each F1 progeny was as low as 20 - 50 eggs. Most eggs were variable in shape, and their proliferation was arrested in early stages. Some eggs did not have a hard shell and hence were probably unfertilized. These results suggest that the F1 progenies that grew to adults escaped the effect of RNAi by using the CeRDH1 protein synthesized before the dsRNA injection. If this is the case, then it is not surprising that all the F2 eggs died, because the F1 escapers could not synthesize CeRDH1 protein due to RNAi. This phenotype was equally observed in worms injected into either the gonads or the intestinal cytoplasms. The chromosome structure and The shape of some oocytes of both I0 and F1 were irregular and variable compared with oocytes of control hermaphrodite. The variability in the roundness of the eggs that died in the early stages due to RNAi with Ce-rdh-1 may be a result of the heterogeneous shape of oocytes in gonads. Moreover, the chromosome structure of oocytes was drastically changed in F1 escapers. On the other hand, no significant difference in chromosome structure was observed between the F1 and control worms during mitosis or in the early prophase of meiosis I. In all the oocytes of F1 escapers, the chromosome condensation of six bivalents and the dissociation of chiasmata were blocked. Meiosis in normal oocytes pauses in the diakinesis stage and the six condensed bivalents are visualized and counted. The oocyte nuclei do not go on to complete meiotic divisions I and II until ovulation and fertilization have occurred. These results indicate that oogenesis in the F1 escapers could not enter the diakinesis stage.
• Remark: paper remark: Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation. CDNA clone yk401c3 was used as template for dsRNA.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004297	rad-51	Y43C5A.6	Caenorhabditis elegans

3 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:Y43C5A.6a	Y43C5A.6a	1188	IV: 10281976-10282102
CDS:Y43C5A.6b	Y43C5A.6b	1074	IV: 10281976-10282102
CDS:Y43C5A.6c	Y43C5A.6c	1089	IV: 10281976-10282102

0 Laboratories

6 Phenotype

Identifier	Name	Description
WBPhenotype:0000154	reduced brood size	The characteristic number of offspring produced is lower compared to that of control animals.
WBPhenotype:0001100	early embryonic lethal	Animal dies prior to the gastrulation stage of embryogenesis.
WBPhenotype:0001362	chromosome condensation failure	The progressive compaction of dispersed interphase chromatin into threadlike chromosomes prior to mitotic or meiotic nuclear division, or during apoptosis in eukaryotic cells is abolished.
WBPhenotype:0001836	eggshell morphology variant	Any variation in the overall structure or appearance of the protective barrier which isolates the embryo from the external environment, compared to control .
WBPhenotype:0000373	egg shape variable	The overall structure or appearance of fertilized oocytes that are laid exhibit nonconsistent and significant deviation from that of control animals.
WBPhenotype:0001898	meiotic sister chromatid segregation variant	Variations in the process by which sister chromatids, identical chromosomes resulting from DNA replication, are physically separated during the meiotic cell cycle, compared to control.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00003358

1 Strain

WormBase ID
WBStrain00000001