WormMine: RNAi WBRNAi00106258

• WormBase ID: WBRNAi00106258
• Genotype: Phsp16-2::prkl-1
• Phenotype Remark: prkl-1 RNAi induction at the mid-L4 stage, after VC4 and VC5 neurons had undergone normal polarizationand begun laterally-directed neurite extension along the vulva, resulted in the emergence of new A/P-directed neuritis. These findings strongly support the notion that active PCP-like signaling or at least PRKL-1 activity is required persistently during development to maintain neuronal polarity.
• Remark: Temporal control of prkl-1 knock-down was achieved using an extrachromosomal transgene bearing sense and anti-sense prkl-1 sequences under the control of the hsp16-2 heat shock-inducible promoter (Phsp16-2::prkl-1).

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00022727	prkl-1	ZK381.5	Caenorhabditis elegans

2 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:ZK381.5b	ZK381.5b	1230	IV: 6979028-6979141
CDS:ZK381.5a	ZK381.5a	1572	IV: 6979028-6979141

0 Laboratories

1 Phenotype

Identifier	Name	Description
WBPhenotype:0000104	cell polarity variant	Contents or structures of a cell are no longer restricted/localized to their typical positions within the cell.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00040178

0 Strain