WormMine: RNAi WBRNAi00116230

• WormBase ID: WBRNAi00116230
• Phenotype Remark: Authors used an established in vivo protein degradation assay to test the turnover of an engineered fluorescently labeled model substrate. Authors monitored endoplasmic reticulum (ER)-associated protein degradation (ERAD). They followed the turnover of an unstable form of the cathepsin L-like cysteine protease CPL-1 (CPL-1-YFP), exclusively expressed in intestinal cells by the nhx-2 promoter. Under standard conditions, CPL-1-YFP is retro-translocated out of the ER lumen for proteasomal degradation in the cytosol. CPL-1-YFP levels were increased in animals treated with alg-2 (RNAi), indicating a decrease in endoplasmic reticulum (ER)-associated protein degradation.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000106	alg-2	T07D3.7	Caenorhabditis elegans

2 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:T07D3.7a	T07D3.7a	2733	II: 873212-873266
CDS:T07D3.7b	T07D3.7b	2676	II: 874162-874262

0 Laboratories

1 Phenotype

Identifier	Name	Description
WBPhenotype:0001806	protein stabilization variant	Any variation in the ability of a protein to resist disintegration via chemical or physical modifications compared to control.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00061892

0 Strain