Transcripts that showed significantly decreased expression in male B. malayi 120 days after infecting gerbils, comparing to 30 days after infecting gerbils.
Proteins that showed significantly increased expression in parasites exposed to 20-hydroxyecdysone.
An empirical filter of proteins with more than two peptides identified, a P value <0.05 and a greater than two-fold change of differential expression, was applied to the data. The Bonferroni correction was applied to correct the P value for the proteins analyzed.
Time-course transcript abundance profiles. P116, k-means clusters of Brugia malayi during development in Aedes aegypti LVP between day 1 and 8 post infection; H114, k-means clusters of Ae. aegypti LVP infected with B. malayi between day 0 and 8 post infection; and HR14, host response profile comparing infected vs. uninfected A. aegypti LVP.
Non-flat profiles (p < 0.01 and maximum fold-difference among time points > 2) were grouped into common temporal patterns using k-means clustering.