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Expr12011
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The earliest Ppa-obi-1 expression occurs in at least one cell in the pre-comma embryonic stage but soon disappears until weak expression reappears in the hyp7 and seam cells in J4 larvae. The most robust and diverse Ppa-obi-1 expression was found in mid-J4 hermaphrodites, which is consistent with real time quantitative PCR results indicating transcript levels in J2 and J3 larvae are less than in J4 larvae. In the mid-J4 stage, Ppa-obi-1 is strongly expressed in the major hypodermal syncytium and the lateral hypodermal cells that cover most of the body (hyp7 and seam cells) as well as the ventral tail Ppa-obi-1 is also strongly expressed in the P(5-7).p cell descendants that form the vulva lumen (vulC, D, E) during the mid-J4 stage until just prior to vulval eversion, as well as in the vulva muscles (likely vm1, 2). Most notably, Ppa-obi-1 expression was observed in the two bilaterally symmetrical specialized epithelial cells that envelope the chemosensory neurons, known as the amphid sheath cells. Ppa-obi-1p::gfp expression in the putative amphid sheath cells lie dorsal-lateral and posterior to the DiI labeled amphid neurons ASK, ADL, and ASI. Expression was also noticed in four unknown cell types anterior to the metacarpus (anterior pharyngeal bulb), which could be putative neuronal support cells for the inner labial or outer labial quadrant sensilla (IL or OLQ). Ppa-obi-1 is also expressed in putative duct and excretory cells on the ventral anterior side that form part of the excretory system. |
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