WormMine: ExpressionPattern Expr12011

• Pattern: The earliest Ppa-obi-1 expression occurs in at least one cell in the pre-comma embryonic stage but soon disappears until weak expression reappears in the hyp7 and seam cells in J4 larvae. The most robust and diverse Ppa-obi-1 expression was found in mid-J4 hermaphrodites, which is consistent with real time quantitative PCR results indicating transcript levels in J2 and J3 larvae are less than in J4 larvae. In the mid-J4 stage, Ppa-obi-1 is strongly expressed in the major hypodermal syncytium and the lateral hypodermal cells that cover most of the body (hyp7 and seam cells) as well as the ventral tail Ppa-obi-1 is also strongly expressed in the P(5-7).p cell descendants that form the vulva lumen (vulC, D, E) during the mid-J4 stage until just prior to vulval eversion, as well as in the vulva muscles (likely vm1, 2). Most notably, Ppa-obi-1 expression was observed in the two bilaterally symmetrical specialized epithelial cells that envelope the chemosensory neurons, known as the amphid sheath cells. Ppa-obi-1p::gfp expression in the putative amphid sheath cells lie dorsal-lateral and posterior to the DiI labeled amphid neurons ASK, ADL, and ASI. Expression was also noticed in four unknown cell types anterior to the metacarpus (anterior pharyngeal bulb), which could be putative neuronal support cells for the inner labial or outer labial quadrant sensilla (IL or OLQ). Ppa-obi-1 is also expressed in putative duct and excretory cells on the ventral anterior side that form part of the excretory system.
• Primary Identifier: Expr12011

11 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
muscle associated with hermaphrodite vulva.	vulval muscle		WBbt:0005821
a structural (glial) cell which forms an specialized environment surrounding the sensory ending(s) of one or more neurons; sometimes accompanied by a more distal socket cell. In early development of the sensory nerves and of the nerve ring some sheath cells (cephalics and labials) may also provide a substrate for axon guidance	neuronal sheath cell	pocket cell	WBbt:0005811
H-shaped cell associated with the excretory system, largest cell in C. elegans.	excretory cell	excretory canal cell	WBbt:0005812
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
entire syncytium of hyp7	hyp7 syncytium	hyp7	WBbt:0005734
The extracellular sinus formed by the excretory duct and pore cells.	Excretory duct		WBbt:0005777
adult vulval toroid cell, E, formed by fusion of precursors of lineage names P6.paal, P6.paar, P6.pppl and P6.pppr.	vulE		WBbt:0006767
adult vulval toroid cell, C, formed by fusion of precursors of lineage names P5.ppal, P5.ppar, P7.papl and P7.papr.	vulC		WBbt:0006765
adult vulval toroid cell, D, formed by fusion of precursors of lineage names P5.ppp and P7.paa.	vulD		WBbt:0006766
cell that exists in and is part of an embryo.	embryonic cell		WBbt:0007028
sheath cell of amphid sensillum.	amphid sheath cell		WBbt:0006754

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00090091	Ppa-obi-1	PPA00537	Pristionchus pacificus

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The fourth stage larva of nematodes.		L4 larval stage	WBls:0000109
	The whole period of embryogenesis in a nematode, from the formation of an egg until hatching.		nematode embryo	WBls:0000102