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Genome-wide analysis of developmental and sex-regulated gene expression profile. |
self-organizing map |
cgc4489_group_18
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Genes from N2 animals with significantly increased expression after 72 hours of treatment on growth media with 10uM rapamycin in 2% DMSO. |
Analysis of gene expression data was carried out with the Affymetrix Transcriptome Analysis Console. Data preprocessing (using RMA normalization) and QC metrics were performed using Affymetrix Expression Console TM and manually inspected afterwards. Expression analysis was carried out for each two pairwise conditions. FDR statistical correction for multiple testing resulted in a slightly lower number of DEGs in most cases. P-value < 0.05 and fold change > 2.0 were used to determine differentially expressed genes. |
WBPaper00048989:N2_rapamycin_upregulated
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Genes up regulated in crh-1(nn3315) comparing to in N2. |
To identify genes that were significantly differentially expressed between each mutant and the control, linear modelling and empirical Bayes analysis was performed using the limma package. Limma computes an empirical Bayes adjustment for the t-test (moderated t-statistic), which is more robust than the standard two-sample t-test comparisons. To correct for multiple testing, Benjamin and Hochbergs method to control for false discovery rate was used. Genes with an adjusted P value of 0.05 or smaller and a fold-change in expression larger than twofold were considered differentially expressed. |
WBPaper00038172:crh-1null_up_regulated
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Transcripts that showed significantly increased expression in scc-1(ubs19);coh-1(ubs26) comparing to in control animals. |
DESeq2 v.1.34, fold change > 2, FDR < 0.05. |
WBPaper00067078:scc-1(ubs19);coh-1(ubs26)_upregulated
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mixed spermatogenesis/somatic |
Comparisons were made between genotypes by subtracting the mean log value of one ratio from another, and the significance of the difference was evaluated using Student t-test for two populations. For the fem-3(gf) versus fem-1(lf) direct comparison, authors performed the same analysis, except they used a Students t-test for one population. Author chose a combination of a twofold difference with a t value exceeding 99% confidence (P < 0.01), because these criteria allowed the inclusion of essentially all genes that had previously been identified as germline-enriched in a wt/glp-4 hermaphrodite comparison. Additionally, requiring a twofold difference reduced false positives, as the number of genes with two-fold difference and a P<0.01 only included ~100 genes more than with P < 0.001, and almost all genes showed germline expression by in situ hybridization. |
[cgc6390]:mixed_spermatogenesis-somatic
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Transcripts that showed significantly increased expression in nath-10(icb102) comparing to in N2 at L2 larva stage. |
Differences in gene expression were calculated using the negative binomial test in the DESeq package (FDR = 0.1). |
WBPaper00061466:nath-10(icb102)_upregulated
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Transcripts that showed significantly increased expression in nath-10(icb99) comparing to in N2 at L2 larva stage. |
Differences in gene expression were calculated using the negative binomial test in the DESeq package (FDR = 0.1). |
WBPaper00061466:nath-10(icb99)_upregulated
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Bacteria infection: Yersinia pseudotuberculosis YP III |
Genes that significantly decreased expression 1 our after biofilm was formed by Y. pseudotuberculosis YP III comparing to worms infected by Y. pseudotuberculosis 3384 mutant (that do not form biofilm) |
Results are presented as the relative up- or downregulation for genes with a difference of 2 fold or more after analyzing expression and using confidence limits with multiple testing corrections (Benjamini-Hochberg FDR). |
WBPaper00045865:biofilm_downregulated_A
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