WormMine

WS295

Intermine data mining platform for C. elegans and related nematodes

Gene :

WormBase Gene ID  ? WBGene00306000 Gene Name  T12B5.18
Sequence Name  ? T12B5.18 Organism  Caenorhabditis elegans
Automated Description  Is affected by sir-2.1; lin-14; and lin-4 based on microarray studies. Biotype  SO:0000336
Genetic Position  III:± Length (nt)  ? 483
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1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Synonyms

Value
WBGene00306000

Genomics

0 Transcripts

 

Other

0 CDSs

0 RNAi Result

47 Allele

Public Name
gk963623
gk962532
gk964281
WBVar02120666
WBVar02124686
WBVar01322894
WBVar01322896
WBVar02124779
WBVar02121583
WBVar02124401
WBVar02123377
WBVar02122933
WBVar02121974
WBVar02124343
WBVar02123578
WBVar02122810
WBVar02121404
WBVar02122559
WBVar02120832
WBVar02122116
WBVar02124101
WBVar02121158
WBVar02122375
WBVar02121865
WBVar02121742
WBVar02123217
WBVar02124181
WBVar02122453
WBVar02123672
WBVar02120984

1 Chromosome

WormBase ID Organism Length (nt)
III Caenorhabditis elegans 13783801  

1 Chromosome Location


Feature . Primary Identifier
Start End Strand
WBGene00306000 947784 948266 -1

2 Data Sets

Name URL
WormBaseAcedbConverter  
C. elegans genomic annotations (GFF3 Gene)  

1 Downstream Intergenic Region

WormBase ID Name Sequence Name Length (nt) Chromosome Location Organism
intergenic_region_chrIII_946301..947783   1483 III: 946301-947783 Caenorhabditis elegans

7 Expression Clusters

Regulated By Treatment Description Algorithm Primary Identifier
  Genes found to be regulated by low-copy overexpression of sir-2.1 with p < 0.014. N.A. WBPaper00026929:sir-2.1_overexpression_regulated
  Genes expressed in N2. Expressed transcripts were identified on the basis of a Present call in 3 out of 4 N2 experiments as determined by Affymetrix MAS 5.0. WBPaper00025141:N2_Expressed_Genes
  Genes expressed in embryonic motor neurons (identified by unc-4::GFP expressing cells). Genes called Present by MAS 5.0 in 2 out of 3 unc-4::GFP hybridizations. WBPaper00025141:unc-4::GFP_Expressed_Genes
  Class F gene expression showed no change in lin-14(lf) in L1, down regulation in lin-4(lf) in L2. Raw data from each experiment were downloaded from the Stanford Microarray Database into Excel files and processed as follows: (i) sort by Spot Flag and discard any rows where the Spot Flag value was nonzero, indicating a bad PCR; (ii) sort by Failed and discard any rows where the Failed value was nonzero, indicating abnormal hybridization; (iii) import into a common file for each type of experiment (i.e., lin-14 or lin-4) the columns from each raw experimental file [RAT2(R/G), which shows a log base 2 transformed ratio of normalized red/green signal for each spot; name of spot (Wormbase designation); chromosome location and description (www.wormbase.org)]; (iv) calculate an average RAT2(R/G) based on the 2 or 3 values (avg; any rows which had only one good experimental value were discarded); (v) calculate a standard deviation (stdev) for the average value; (vi) calculate a t value for each spot by using the formula t = avg*[sqrt(n - 1)]/stdev, where n is the number of experiments for which good data exist, sqrt is square root, and stdev is standard deviation; (vii) sort by absolute t value and discard any rows with a t value below 4.303 (below 95% confidence interval for three experiments) or below 12.706 (below 95% confidence interval for two experiments); (viii) sort by absolute average value and discard any rows with average values below 1.0 (less than twofold change compared to control). WBPaper00026952:class_F
Bacteria infection: Serratia marcescens Caenorhabditis elegans Genes with expression levels changed significantly after treatment of Serratia marcescens. Differential expression were calculated by empirical eBayes method using eBayes function. P_value <= 0.01 and log2 fold change > 1 were used to call differentially expressed genes in all datasets. WBPaper00041606:CE_S.marcescens_regulated
  Genome-wide analysis of developmental and sex-regulated gene expression profile. self-organizing map cgc4489_group_21
Bacteria infection: Enterococcus faecalis Genes up-regulated in animals infected with Enterococcus faecalis compared to the E. coli OP50 control after 24h of infection. MAANOVA and BRB-Array-Tools. WBPaper00030985:Enterococcus_faecalis_upregulated

0 Expression Patterns

0 GO Annotation

0 Homologues

1 Locations


Feature . Primary Identifier
Start End Strand
WBGene00306000 947784 948266 -1

0 Ontology Annotations

0 Regulates Expr Cluster

1 Sequence

Length
483

1 Sequence Ontology Term