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Germline-enriched and sex-biased expression profile cluster C. |
hierarchical clustering |
[cgc6390]:Cluster_C
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Transcripts that showed significantly decreased expression in animals after 24 hour exposure to 100ug per ml silica nanoparticles (SiNPs). |
The random variance model (RVM)t-test was applied toidentify differentially expressed mRNAs and miRNAsbetween the SiNPs-treated group and the control group. P-value <0.05 and fold change (FC) >1.5 were considered as the threshold screening between the SiNP-treated groupand the control group. |
WBPaper00060100:SiNP_downregulated_mRNA
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Transcripts that showed significantly decreased expression in ifo-1(kc2) comparing to in N2 animals. |
ANOVA, p-value < 0.05. |
WBPaper00056167:ifo-1(kc2)_downregulated
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male sex-enriched |
Comparisons were made between genotypes by subtracting the mean log value of one ratio from another, and the significance of the difference was evaluated using Student t-test for two populations. For the fem-3(gf) versus fem-1(lf) direct comparison, authors performed the same analysis, except they used a Students t-test for one population. Author chose a combination of a twofold difference with a t value exceeding 99% confidence (P < 0.01), because these criteria allowed the inclusion of essentially all genes that had previously been identified as germline-enriched in a wt/glp-4 hermaphrodite comparison. Additionally, requiring a twofold difference reduced false positives, as the number of genes with two-fold difference and a P<0.01 only included ~100 genes more than with P < 0.001, and almost all genes showed germline expression by in situ hybridization. |
[cgc6390]:male_sex-enriched
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Genome-wide analysis of developmental and sex-regulated gene expression profile. |
self-organizing map |
cgc4489_group_20
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Bacteria infection: Pseudomonas aeruginosa PA14 |
Genes up regulated by P. aeruginosa Infection. |
Two-way ANOVA |
WBPaper00028482:PA14_upregulated
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