WormMine: Gene WBGene00194831

• WormBase Gene ID: WBGene00194831
• Gene Name: mir-2220
• Sequence Name: Y59E1B.4
• Organism: Caenorhabditis elegans
• Automated Description: Is affected by alg-5 based on RNA-seq studies. Is affected by nicotine; multi-walled carbon nanotube; and sanguinarine based on quantitative PCR and RNA-seq studies.
• Biotype: SO:0001265
• Genetic Position: X :-17.4339±
• Length (nt): 58

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Synonyms

• Value: WBGene00194831

Genomics

2 Transcripts

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
Transcript:Y59E1B.4a	Y59E1B.4a	22	X: 1873827-1873848
Transcript:Y59E1B.4b	Y59E1B.4b	22	X: 1873863-1873884

Other

0 CDSs

1 RNAi Result

• WBRNAi00057767

6 Allele

• gk963725
• gk963864
• gk964444
• gk964445
• gk963977
• gk676035

1 Chromosome

• WormBase ID: X
• Organism: Caenorhabditis elegans
• Length (nt): 17718942

1 Chromosome Location

• Feature . Primary Identifier: WBGene00194831
• Start: 1873827
• End: 1873884
• Strand: 1

2 Data Sets

• WormBaseAcedbConverter
• C. elegans genomic annotations (GFF3 Gene)

0 Downstream Intergenic Region

7 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	MicroRNAs that showed significantly decreased expression in alg-5(ram2), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-5(ram2)_downregulated_miRNA
	miRNA that showed increased expression after 20mM nicotine treatment	Statistical analysis was based on t-test for independent samples via SPSS. The results were further narrowed based on two criteria. Only the genes whose expression changed with a P < 0.05 and a fold change > 0.5, when compared to control, were considered as differentially expressed. Fold change was calculated as 2**(DeltaCt(treatment)-DeltaCt(control))-1. DeltaCt = Ct(miRNA)-Ct(aveMRNA).	WBPaper00042480:20mM_nicotine_upregulated
	MicroRNAs that showed significantly decreased expression in alg-5(tm1163), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-5(tm1163)_downregulated_miRNA
	MicroRNAs that showed significantly decreased expression after animals were exposed to 1 mg per liter multi-walled carbon nanotubes (MWCNTs) from L1 larva to young adult.	DESeq, cut-off of 2-fold change.	WBPaper00045019:MWCNT_downregulated
	Micro RNAs that showed significantly lower expression (log2fold > 2, padj < 0.01) in hermaphrodite gonad (somatic gonad plus germine) than in male gonad (somatic gonad plus germine).	DESeq2 v1.13.8, fold change > 2, FDR < 0.01.	WBPaper00056161:male_enriched_miRNA
	miRNAs in F2 generation down regulated by exposure to 20uM and 20mM nicotine during F0 generation.	Statistical analysis was based on t-test for independent samples via SPSS to compare each of the nicotine treatments with control (i.e. low vs. control and high vs. control). Welch test correction was performed to account for unequal variance in each of the compared groups (control and treatment). In addition, to increase the statistical stringency, miRNAs differential expression was deemed significant only if it fulfilled two criteria: p value , 0.05 and expression changed by at least 50% relative to control.	WBPaper00049006:nicotine_downregulated_F2
	miRNAs that showed significantly increased expression after 48 hour treatment in 10uM sanguinarine.	Fold change > 2.	WBPaper00055663:sanguinarine_upregulated

0 Expression Patterns

0 GO Annotation

0 Homologues

1 Locations

• Feature . Primary Identifier: WBGene00194831
• Start: 1873827
• End: 1873884
• Strand: 1

0 Ontology Annotations

0 Regulates Expr Cluster

1 Sequence

• Length: 58

1 Sequence Ontology Term