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Transcripts that showed significantly increased expression in hpl-2(tm1489) comparing to in N2 animals. |
DESeq2, adjusted p-value < 0.05, log2 fold change > 2 or < -2. |
WBPaper00054493:hpl-2(tm1489)_upregulated
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Potental DAF-12 target genes identified by ChIP-chip analysis performed on strain ALF4 [daf-12 |
Affymetrix TAS software that computed for each probe estimates of fold enrichment (in linear scale) over hybridization with input DNA. At the same time, TAS calculated for each probe a p-value by applying a Wilcoxon signed rank test. A threshold of 2.5 was selected, which corresponds to probe intensities approximately 2.5 times stronger on the ChIP array than on the Input array. Additional TAS threshold parameters were MinRun=180 bp, MaxGap=300 bp. TAS analysis showed that the selected threshold of 2.5 corresponds approximately to a p-value of 0.01. |
WBPaper00040221:DAF-12_target_ALF4
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Transcripts that showed significantly altered expression in olrn-1(ums9) comparing to in N2 animals. |
The Ballgown package from the Bioconductor software suite (version 3.8) was used to run a custom R script in R console(R Version 3.5) to analyze the differential gene expression, visualize the data, and perform statistical tests for differential expressionwith multiple test correction. A gene was considered to be differentially regulated if its fold change versus wild-type was greater thantwo, the adjusted p value was less than 0.05, and its RPKM was greater than one. |
WBPaper00059567:olrn-1(ums9)_regulated
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Transcripts that showed significantly increased expression in VC1743[mett-10(ok2204)] comparing to in N2 when animals grow on standard (OP50) plates. |
DESeq2, p-value < 0.05, fold change > 2. |
WBPaper00061357:mett-10(ok2204)_upregulated_OP50
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