WormMine: AnatomyTerm WBbt:0004292

• Definition: a single, H-shaped cell which lies just above the anus and connects the roof of the anal canal to the dorsal bodywall; its contractions act to increase the size of the anal opening by lifting the roof of the rectum and hence facilitate expulsion of intestinal contents.
• Name: anal depressor muscle
• Primary Identifier: WBbt:0004292
• Synonym: dilator muscle

2 Children

Definition	Name	Synonym	Primary Identifier
Anal depressor muscle of a male animal.	anal depressor muscle male		WBbt:0008385
Anal depressor muscle of a hermaphrodite animal.	anal depressor muscle hermaphrodite		WBbt:0008386

9 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Top 300 transcripts enriched in intestinal muscle, anal depressor muscle according to single cell RNAseq.	Top 300 enriched transcripts were determined by log2.ratio of the tpm in the cell type vs the tpm in the other cells * the log2 of the cell.type tpm.	WBPaper00061340:mu_int_mu_anal
	Single-cell RNA-Seq cell group 7_2 expressed in muscle.	scVI 0.6.0	WBPaper00065841:7_2
	Single-cell RNA-Seq cell group 7_4 expressed in muscle.	scVI 0.6.0	WBPaper00065841:7_4
	Single-cell RNA-Seq cell group 38_3 expressed in muscle.	scVI 0.6.0	WBPaper00065841:38_3
	Single-cell RNA-Seq cell group 7_3 expressed in muscle.	scVI 0.6.0	WBPaper00065841:7_3
	Enriched in anal depressor muscle (adult).		WBPaper00029359_1692
	Single-cell RNA-Seq cell group 38_1 expressed in muscle.	scVI 0.6.0	WBPaper00065841:38_1
	Single-cell RNA-Seq cell group 38_2 expressed in muscle.	scVI 0.6.0	WBPaper00065841:38_2
	Enriched in body wall muscle (adult), vulval muscle (adult), anal depressor muscle (larva).		WBPaper00029359_1636

550 Expression Patterns

• Primary Identifier: Expr4870
• Pattern: A-class motor neuron: expressed in embryo; enriched in larva (1.6). Neuronal expression include: All ventral cord motor neurons, head neurons, touch neurons. Also expressed in other cells: Intestine, anal depressor, head muscle. Pan-neuronal: expressed in embryo and larva.

• Primary Identifier: Expr4852
• Pattern: A-class motor neuron: enriched in larva (1.8); not expressed in embryo. Neuronal expression include: All ventral cord motor neurons, head and tail neurons, touch neurons. Also expressed in other cells: Body muscle, anal depressor, sphincter muscle? Excretory gland cells?. Pan-neuronal: enriched in larva (3.2); not expressed in embryo.

• Primary Identifier: Expr4841
• Pattern: A-class motor neuron: expressed in embryo; not expressed in larva. Neuronal expression include: All ventral cord motor neurons, head and tail neurons, PLN. Also expressed in other cells: Pharyngeal muscle, vulval muscle, anal depressor, hypodermis. Pan-neuronal: enriched in embryo (1.8); not expressed in larva.

• Primary Identifier: Expr4840
• Pattern: A-class motor neuron: expressed in embryo; not expressed in larva. Neuronal expression include: All ventral cord motor neurons, head and tail neurons, touch neurons. Also expressed in other cells: Intestine, vulval muscle, pharyngeal muscle, anal depressor, body muscle. Pan-neuronal: enriched in embryo (1.8); not expressed in larva.

• Primary Identifier: Expr4820
• Remark: Picture: Figure S1.
• Pattern: Expressed in head neurons, ventral-cord motor neurons, body-wall muscle, vulval muscle, stomatointestinal muscle, anal-depressor muscle, tail neurons, etc.

• Primary Identifier: Expr4819
• Remark: Picture: Figure S1.
• Pattern: Expressed in head neurons, ventral-cord motor neurons, body-wall muscle, vulval muscle, stomatointestinal muscle, anal-depressor muscle, tail neurons, etc.

• Primary Identifier: Expr4809
• Remark: Picture: Figure 8. Staining was greatly reduced in unc-87(e843).
• Pattern: The antibody react with body wall muscle, pharyngeal muscle, anal depressor and sphincter muscle, intestinal muscles, vulval muscles and uterine muscles. In all cases, UNC-87 was contained withing the pattern observed with phalloidin or anti-actin antibodies, suggesting colocalization with thin filaments..
• Subcellular Localization: Immunohistochemical staining of wild type adult body wall muscle revealed a atriated pattern. The striations are interupted periodically along their length by unstained regions. Double labelling experiments showed that anti-UNC-87 staining pattern corresponded with that of the monoclonal antibody MH44. This pattern represent the I band. At the center of the I band are the dense bodies, these correspond to the unstained regions within the striations.

• Primary Identifier: Expr4742
• Pattern: The anti-CeTNI-2 antibody strongly stained the body wall muscle, and faintly the pharyngeal muscle, vulval muscles and anal muscles.
• Subcellular Localization: At high magnification the anti-CeTNI-2 antibody stained the thin filaments in the I-band regions that also contain actin.

• Primary Identifier: Expr4740
• Remark: Reporter gene fusion type not specified.
• Pattern: The tni-2 and tni-3 genes were also expressed in the same cells of body wall, vulval and anal muscles, but while tni-2 was uniformly expressed in body wall muscles, the tni-3 gene was strongly expressed in the head, vulval and anal muscles. Expression of tni-2::gfp was observed in the vulval and anal muscles in addition to those muscles detected with tni-2::lacZ. This was the result of the inclusion of a longer 5' upstream region of tni-2 that includes the NdE-box enhancer for vulval expression.

• Primary Identifier: Expr4741
• Remark: Reporter gene fusion type not specified.
• Pattern: The tni-2 and tni-3 genes were also expressed in the same cells of body wall, vulval and anal muscles, but while tni-2 was uniformly expressed in body wall muscles, the tni-3 gene was strongly expressed in the head, vulval and anal muscles. Expression of tni-2::gfp was observed in the vulval and anal muscles in addition to those muscles detected with tni-2::lacZ. This was the result of the inclusion of a longer 5' upstream region of tni-2 that includes the NdE-box enhancer for vulval expression.

• Primary Identifier: Expr4734
• Pattern: A transcriptional arg-1::gfp reporter is expressed in the head mesodermal cell, the vulval muscles, and the enteric muscles.

• Primary Identifier: Expr4994
• Remark: Picture: Figure 1B, Figure S3A.
• Pattern: The unc-80 GFP reporter is broadly expressed in the nervous system. The reporter is expressed in both acetylcholine and GABA motor neurons as determined by double-labeling. Although expression is observed in other tissues, no expression was observed in the body muscle. unc-80 is strongly expressed in neurons. Strong expression is observed in the nervous system and in the vulval muscle. Expression was also shown in the anterior excretory canal just posterior of the head, the vulval muscle, distal tip cell of the gonad, and the anal depressor muscle in the tail.

• Primary Identifier: Expr4942
• Pattern: strong bwm, vul, anal dep, mu int; strain A shows embryonic bwm starting at 1.5 fold and strong at 3-fold

• Primary Identifier: Expr4943
• Pattern: strong 3-fold emb bwm that fades and becomes mosaic in late L's and adults, vul, anal dep, spinchter, mu int; seam cells in one line at least

• Primary Identifier: Expr4944
• Pattern: strong bwm, vulval muscle and anal depressor, strong bwm at 3-fold into L1s, earliest exp is faint bwm in 2-fold seen in line B.

• Primary Identifier: Expr4930
• Pattern: strong bwm, vul, anal, rare ant pharynx, bwm precursors, tail hyp, neurons; early embryonic bwm at least by horseshoe stage

• Primary Identifier: Expr4931
• Pattern: strong bwm, pharyngeal, vulval, and anal depressor; a bit mosaic; starts at 3-fold?

• Primary Identifier: Expr4932
• Pattern: strong anal dep, sphinct, vul,; moderate and sporadic bwm; no embryonic seen; re-checked embryonic 6-15 and saw none.

• Primary Identifier: Expr4934
• Pattern: strong bwm, pharyngeal, vul, anal, mu int, sphincter muscles; no embryonic seen

• Primary Identifier: Expr4920
• Pattern: Faint vul, sphincter, anal dep, mu int; faint head neurons; rare spermatheca; bean to L1 see head bwm?- 4 quads of single to double cells GFP+ that don't exactly look like bwm close to bwm position - could be unhappy bwm due to exp?

• Primary Identifier: Expr4924
• Pattern: strong GFP expression in BWM, anal muscle and vulval muscles. GFP visible in late embryo.

• Primary Identifier: Expr4918
• Pattern: Strong vul, bwm, anal, mu int, sphincter, head neurons.

• Primary Identifier: Expr4919
• Pattern: Strong pharyngeal, vulval, bwm, anal dep, mu int; 3-fold earliest embryonic.

• Primary Identifier: Expr4911
• Remark: Picture: Fig S5.
• Pattern: A transcriptional fusion of the unc-50 promoter to GFP is expressed in all cell types throughout development. unc-50::gfp expression was seen in the head of adult hermaphrodite. Expression is seen in the pharyngeal muscle, head neurons, and epidermis. Expression is also present in the gut, ventral cord motor neurons, and epidermal seam cells. In the mid body region expression is seen in the gut, seam cells, uterus, vulva muscles, and distal tip cell. In the tail region unc-50 expression can be detected in the epidermis and enteric muscles.

• Primary Identifier: Expr4914
• Pattern: Strong GFP expression in BWM, anal muscle and vulval muscles. GFP visible in late embryo.

• Primary Identifier: Expr4382
• Pattern: Expressed in AVA, RIB, RID, SIB, SMD, CEP(?), ADE(?), some unidentified neurons in the head and tail. Also expressed in anal depressor, head muscles (strong), body wall muscles (strong).

• Primary Identifier: Expr4388
• Remark: Gene_regulation: Animals bearing the plasmids with CE1(bs258), pC46H11BS1888 and pC46H11BB1439 showed gfp expression in body-wall muscles, the vulval muscles and anal depressor muscle, whereas animals with the plasmids pC46H11BB1164 and pC46H11BE1281, which does not contain CE1(bs258), showed no to little expression in these tissues. CE1(bs258) is located in the first intron of C46H11.6. In gfp and lacZ reporter gene assays the CE1(bs258) element appeared to behave as an enhancer element for the expression of C46H11.6 but no effect on the expression of the opposite direction gene, pat-10.
• Pattern: Expressed in body-wall muscles, the vulval muscles and anal depressor muscle.

• Primary Identifier: Expr4379
• Remark: No detailed description on life stages.
• Pattern: ZK795.3::GFP expression pattern includes spermatheca, hypodermal cells, pharynx and the excretory cell and channels. In the L3 stage, expression was seen in the vulva, and in P6.p descendants.

• Primary Identifier: Expr4350
• Pattern: Localized differently within cells and GFP fluorescence was seen in body wall muscle, distal tip cells, enteric muscle and cells of the posterior intestine. Within the pharynx, cca-1 expression is observed in most if not all pharyngeal muscle cells but is most prominent in those of the procorpus and in pm8, the most posterior cell in the terminal bulb.

• Primary Identifier: Expr4340
• Remark: No detailed description on expression pattern in other life stages.
• Pattern: acr-8::GFP was strongly expressed in all body muscle cells as well as in anal and vulval muscles and ventral cord motor neurons. Expression was visible in the embryo.

0 Life Stages

1 Parents

Definition	Name	Synonym	Primary Identifier
Muscle cell of the alimentary canal, excluding the pharynx.	enteric muscle		WBbt:0008600