WormMine: AnatomyTerm WBbt:0004382

• Definition: Neuron, dopaminergic of postderid sensillum
• Name: PDER
• Primary Identifier: WBbt:0004382
• Synonym: lineage name: V5R.paaa

1 Children

Definition	Name	Synonym	Primary Identifier
nucleus of pedigree V5R.paaa	V5R.paaa nucleus		WBbt:0002600

0 Expression Clusters

88 Expression Patterns

• Primary Identifier: Expr4843
• Pattern: A-class motor neuron: expressed in larva; enriched in embryo (2.0). Neuronal expression include: Bright in head neurons, few tail neurons. weak in all ventral cord motor neurons. Touch neurons, PDE. Also expressed in other cells: Intestine, head muscle, pharyngeal muscle, hypodermis, distal tip cell. Pan-neuronal: expressed in larva; enriched in embryo(3.0).

• Primary Identifier: Expr4648
• Pattern: Transgenic lines generated with the full-length protein fusion construct (for example, ljEx114) expressed TRPA-1:: GFP in the same cells as ljEx107 (see Expr4646), but with some additional cells, including the majority of amphid sensory neurons (for example, ASH, AWA, AWB, ASI and ASK) and the phasmid neurons PHA and PHB. The full-length TRPA-1:: GFP fusion was also expressed in the PVD and PDE in the postdeirid sensilla and the sensory neurons OLQ and IL1. Other neurons in the head and ventral nerve cord also expressed TRPA-1:: GFP.
• Subcellular Localization: The fusion protein was observed at the cilia of sensory neurons, as well as at the cell body.

• Primary Identifier: Expr4523
• Pattern: Expression of DYF-6::GFP is restricted to a few cells from hatching to adulthood. Of particular note, DYF-6::GFP is consistently expressed in the cell bodies, dendritic bodies, and dendritic endings of the phasmid neurons. Expression was also very evident in the dendritic bodies and endings of the amphid sensilla. DYF-6::GFP was faintly and irregularly expressed in the cell bodies of the dye-filling amphid neurons. Expression of DYF-6::GFP was also seen in the hypodermis and in several neuronal cell bodies in the region of the inner labial cell bodies. Finally, expression can be seen in a lateral neuronal cell body in the region of the PDE cell body in older larvae and adults.
• Subcellular Localization: DYF-6::GFP was also expressed in the cell and dendritic bodies of the phasmid neurons in the six dauer larvae (the dendritic bodies were often kinked, as though their linearity had been affected by shrinkage of the body during formation of the dauer state). In four of the animals, DYF-6::GFP indicated that the phasmid neurons had proper dendritic endings, and IFT of the GFP could be easily seen in the endings. In the other two animals, however, DYF-6::GFP did not provide evidence for the existence of dendritic endings for the phasmid neurons, indicating that the endings might occasionally be severely retracted or modified in dauer larvae. For both the amphid and phasmid neurons, the greatest intensity of DYF-6::GFP was in the transition zone between the dendritic bodies and their ciliary endings. Of particular note, anterograde and, to a lesser extent, retrograde movement of GFP particles could be seen along the length of the dendritic endings of both the amphid and phasmid neurons. In the amphid neurons, the particles moved in the anterograde direction over the combined middle and distal segments of the ciliary axoneme at 0.9 +/- 0.1 m/sec. IFT could be seen prior to hatching and throughout postembryonic development and adulthood. IFT of DYF-6::GFP in dauer larvae: Six dauer larvae from SP2730 were picked from plates exhausted of bacteria to NGM plates without bacteria, followed by a further incubation of 3 days. DYF-6::GFP was expressed in the cell bodies and in the dendritic bodies and endings of the dye-filling amphid neurons. Moreover, IFT could be easily seen in the dendritic endings, but there appeared to be fewer GFP particles undergoing IFT in the amphid bundles relative to that seen in non-dauer animals.

• Primary Identifier: Expr16352
• Pattern: We confirmed previous reports that the asic-1 promoter drives expression in the ADE, CEP, PVQ, PDE and PVD neurons (De Stasio et al., 2018; Husson et al., 2012; Voglis & Tavernarakis, 2008) and also observed expression in FLP and ventral cord neurons.

• Primary Identifier: Expr11820
• Pattern: asic-1 is expressed in eight neurons comprising the C. elegans dopaminergic system (the four cephalic neurons; two anterior deirid neurons and two posterior deirid neurons; CEP, ADE, PDE, respectively). Expression is also detected in four additional neurons in the tail, two of which are the bilaterally symmetric PVQ interneurons.
• Subcellular Localization: A full-length ASIC-1::GFP and an amino-terminal ASIC-1N::GFP chimaeras showed prominent punctuate distribution along the processes of dopaminergic neurons, in synapse-rich areas. A DsRED-synaptobrevin fusion colocalizes with both ASIC- 1::GFP and ASIC-1N::GFP in these puncta. Thus, ASIC-1 localizes at presynaptic termini of dopaminergic neurons.

• Primary Identifier: Expr15649

• Primary Identifier: Expr11574
• Pattern: atat-2 is expressed not only in touch receptor neurons but also in a subset of ciliated neurons, namely, PDE, ADE, CEP, and OLQ.

• Primary Identifier: Expr3037
• Pattern: The 5' untranslated region directed expression to a small subset of sensory cells that are ciliated. GFP signal was observed in the multiple ciliated amphid neurons in the head and both ciliated phasmid neurons (PHA and PHB) in the tail. Expression was also detected in other ciliated sensory neurons, including the inner and outer labial neurons and male tail ray neurons. GFP fluorescence was also detected in the midbody PDE ciliated neuron and PQR ciliated tail neuron.

• Primary Identifier: Expr15560

• Primary Identifier: Expr15571

• Primary Identifier: Expr15572

• Primary Identifier: Expr15573

• Primary Identifier: Expr15579

• Primary Identifier: Expr15586

• Primary Identifier: Expr15651

• Primary Identifier: Expr15652

• Primary Identifier: Expr15589

• Primary Identifier: Expr15591

• Primary Identifier: Expr15598

• Primary Identifier: Expr15604

• Primary Identifier: Expr14590
• Pattern: Embryonic expression of exc-7 was first observed at the bean stage. By reverse lineaging with use of SIMI-Biocell software, we confirm the identity of one of the expressing cells at this stage as the excretory canal cell. In L1 animals, broad expression in the head, ventral nerve cord (VNC), and tail was observed. In young adults, expression is notably observed in vulva cells. In the nervous system specifically, expression is observed in many neurons throughout the body, but unlike Drosophila Elav, exc-7::gfp it is not panneuronally expressed. We confirmed previously reported expression in cholinergic VNC MNs, but absence of GABAergic VNC MNs, consistent with previous reports (Fujita et al., 1999; Loria et al., 2003) and consistent with exc-7 functioning in cholinergic, but not GABAergic neurons to control alternative splicing (Norris et al., 2014). exc-7::gfp is also expressed in some non-neuronal cell types, including muscle and hypodermis, but not in the gut. A previous report showed that exc-7 is only transiently and weakly expressed in the excretory cell, which, based on exc-7's excretory mutant phenotype, has puzzled researchers (Fujita et al., 2003). We find that the gfp tagged exc-7 locus is strongly and continuously expressed in the excretory canal cell.

• Primary Identifier: Expr15608

• Primary Identifier: Expr15611

• Primary Identifier: Expr9224
• Pattern: mtm-9::gfp reporter was expressed in a broad range of tissues, including the muscle, intestine, hypodermis and neurons (including the CAN neuron in the mid-body region). Mtm-9 was also expressed in the rectal epithelial cells that are the major source of EGL-20 Wnt.

• Primary Identifier: Expr1438
• Remark: All of the reporter constructs produced the same cell-specific expression pattern as transgenes.
• Pattern: The reporter transgenes express ubiquitously in the early embryo starting at about the 100 cell stage during gastrulation. In late embryogenesis and posthatching, expression is more limited. Strongest expression is observed in migrating cells and growing neurons as these cells undergo movements on the epidermis. At hatching, the reporters express in many neurons throughout the animal, in several cells of the pharynx including some pharyngeal neurons, in the elongated processes of the excretory cells, in the amphid and phasmid sheath and socket cells, in the tail hypodermis, and at later stages in intestine, muscles, vulva, and somatic gonad including the gonad sheath and hermaphrodite distal tip cells. The neurons expressing unc-73 include the PLM, ALM, PDE, HSN, CAN, PHC, and PVN neurons and the ventral cord motorneurons. Expression in the HSNs is absent in early larval stages, but begins late in the second larval stage (L2), precisely when axon outgrowth is initiated from the HSN cell bodies. The Q neuroblasts, Pn neuroectoblasts, sex myoblasts (SMs), and canal associated neurons (CANs) express unc-73 reporters. The left and right Q cells begin to express the GFP reporter as they initiate their migrations along the longitudinal axis of the epidermis during the early first larval (L1) stage, and expression in these cells continues beyond the completion of their first division. The unc-73 reporters express in the Pn cells just before this second phase of movemen. The distal tip cells also express the unc-73/reporters during their migration.

• Primary Identifier: Expr15402

• Primary Identifier: Expr15406

• Primary Identifier: Expr13568
• Pattern: ztf-6::gfp expression is first observed in late embryogenesis (threefold stage). In early larval stages, the ztf-6::gfp-expressing cells include head hypodermal cells, head muscle cells, neurons, and ectodermal blast cells along the body (all P and all V cells) and in the tail. Starting in the L2 stage, additional neurons in P cell-derived ventral cord motor neurons express ztf-6::gfp. Because of the postdeirid loss phenotype of ztf-6 mutants, we examined the postdeirid lineage in more detail. We observe ztf-6 expression in the V5 cell of freshly hatched L1 animals. Upon division of the V5 cell into a posterior and anterior daughter, we observe expression in both the anterior and posterior daughters of the V5 cell. The descendent of the posterior V5.p daughter, V5.pa (the founder of the postdeirid lineage), and V5.pp also continue to express ztf-6::gfp. Within the V5.pa lineage, expression of ztf-6::gfp is retained in the blast cells that generate the glial cells and the PDE and PVD neurons (v5.paaa, V5.papa, V5.paap, V5.papp). No expression is detected at later stages in this lineage.

• Primary Identifier: Expr12508
• Pattern: TSP-17::GFP fusion protein expression was observed in all dopaminergic neurons: it was uniform along axons and dendrites of both dorsal and ventral pairs of CEP neurons, as well as in ADE neurons and in the posterior PDE neurons. Within the cell body, the TSP-17::GFP fusion seems to be excluded from the nucleus, a pattern that is more evident in a 'close-up' image of a PDE neuron, where the signal appears to form a ring-like structure around the nucleus. mCherry aggregates (coming from the pdat- 1::mcherry::let858 39UTR construct, which are not linked to neurodegeneration) form dot-like structures in dendrites and axons (arrows), and the surrounding TSP-17 fluorescent signal suggests plasma membrane expression. TSP- 17 enrichment at the plasma membrane can be observed most prominently in the large cells of the vulva and the sheath cells enclosing the spermatheca. In the spermatheca, TSP-17::GFP expression is also clearly enriched around the nucleus, possibly localizing to the nuclear membrane or endoplasmic reticulum. TSP-17::GFP is also expressed in multiple neurons throughout worm development. For instance, the NSM serotonergic neuron, which is characterized by extensive axon sprouting, shows TSP-17::GFP expression along its entire length. Prominent expression was also observed in the muscles of early stage larvae. Finally expression also appears to be apparent in muscles of the adult head. In summary, the TSP-17::GFP expression indicates that TSP-17 is expressed in dopaminergic neurons.

• Primary Identifier: Expr10708
• Pattern: Expression of the tiam-1 promoter::cfp construct was primarily in neurons. Most if not all neurons expressed the construct, including neurons in the head and tail, the ventral cord commissural motorneurons, the mechanosensory neurons (ALMs, PLMs, AVM, PVM) and the CAN, PDE, and PVD neurons. tiam-1promoter::cfp expression in neurons began in embryos and lasted throughout adulthood. No obvious expression outside of the nervous system was noted. However, tiam-1 might be endogenously expressed in other tissues, as some transcriptional elements might not be present in this transgene.

0 Life Stages

4 Parents

Definition	Name	Synonym	Primary Identifier
Neuron class of two neurons with ciliated endings in the posterior deirid sensilla.	PDE	postdeirid neuron	WBbt:0006747
postdeirid sensillum, right.	postdeirid sensillum right		WBbt:0006970
post-embryonic cell of pedigree V5R.paa	V5R.paa		WBbt:0007465
posterior lateral ganglion, right side.	posterior lateral right ganglion		WBbt:0005463