WormMine: ExpressionPattern Expr14590

• Pattern: Embryonic expression of exc-7 was first observed at the bean stage. By reverse lineaging with use of SIMI-Biocell software, we confirm the identity of one of the expressing cells at this stage as the excretory canal cell. In L1 animals, broad expression in the head, ventral nerve cord (VNC), and tail was observed. In young adults, expression is notably observed in vulva cells. In the nervous system specifically, expression is observed in many neurons throughout the body, but unlike Drosophila Elav, exc-7::gfp it is not panneuronally expressed. We confirmed previously reported expression in cholinergic VNC MNs, but absence of GABAergic VNC MNs, consistent with previous reports (Fujita et al., 1999; Loria et al., 2003) and consistent with exc-7 functioning in cholinergic, but not GABAergic neurons to control alternative splicing (Norris et al., 2014). exc-7::gfp is also expressed in some non-neuronal cell types, including muscle and hypodermis, but not in the gut. A previous report showed that exc-7 is only transiently and weakly expressed in the excretory cell, which, based on exc-7's excretory mutant phenotype, has puzzled researchers (Fujita et al., 2003). We find that the gfp tagged exc-7 locus is strongly and continuously expressed in the excretory canal cell.
• Primary Identifier: Expr14590

197 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
neuron with cell body associated with the ventral nerve cord.	ventral cord neuron	ventral cord motoneuron	WBbt:0005300
female genital.	vulva		WBbt:0006748
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring.	ventral nerve cord	ventral cord	WBbt:0005829
type of cells that make up muscle layers in the pharynx.	pharyngeal muscle cell		WBbt:0005451
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
Neuron class of one sensory neuron, basal body, not part of a sensillum, projects into preanal gangion.	PQR	lineage name: QL.ap	WBbt:0004096
Pharyngeal sensory-motorneurons	m3L	lineage name: ABaraapappp	WBbt:0004471
Pharyngeal sensory-motorneurons	m3R	lineage name: ABaraappppp	WBbt:0004469
Neuron class of eleven motoneurons, with cell bodies in the ventral cord, which innervate dorsal muscles.	AS neuron		WBbt:0005334
Major cell type of nervous tissue, specialized for transmission of information in the form of patterns of impulses.	neuron	neurone	WBbt:0003679
Amphid interneuron	AIYL	lineage name: ABplpapaaap	WBbt:0003963
Amphid interneuron	AIYR	lineage name: ABprpapaaap	WBbt:0003961
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNL	lineage name: ABplapppappa	WBbt:0004758
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNR	lineage name: ABprapppappa	WBbt:0004757
Hermaphrodite specific ventral cord motor neuron innervates vulval muscles and ventral body muscles	VC4	lineage name: P6.aap	WBbt:0004617
Hermaphrodite specific ventral cord motor neuron innervates vulval muscles and ventral body muscles	VC5	lineage name: P7.aap	WBbt:0004613
Neuron class of one pharyngeal motorneuron.	M5 neuron	lineage name: MSpaaapap	WBbt:0004465
Neuron class of one pharyngeal interneuron, posterior sensory.	I5 neuron	I5	WBbt:0004740
Interneuron, associated with PLM	PLNL	lineage name: TL.pppap	WBbt:0004102
Interneuron, associated with PLM	PLNR	lineage name: TR.pppap	WBbt:0004098
Neuron, dopaminergic of postderid sensillum	PDER	lineage name: V5R.paaa	WBbt:0004382
Neuron, dopaminergic of postderid sensillum	PDEL	lineage name: V5L.paaa	WBbt:0004383
neuron of the pharyngeal nervous system.	pharyngeal neuron		WBbt:0005439
Inner labial neuron	IL1VL	lineage name: ABalppapppaa	WBbt:0004549
Inner labial neuron	IL1VR	lineage name: ABarapppppaa	WBbt:0004547
Inner labial neuron	IL1DL	lineage name: ABalapappaaa	WBbt:0004738
Inner labial neuron	IL1DR	lineage name: ABalappppaaa	WBbt:0004737
Inner labial neuron	IL1L	lineage name: ABalapaappaa	WBbt:0004553
Inner labial neuron	IL1R	lineage name: ABalaappppaa	WBbt:0004551

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001368	exc-7	F35H8.5	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 350-390min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. Emrbyo elongation started but have not formed comma shape yet. The shape of embryo looks like a lima bean. A stage right before comma embryo. Also called lima embryo or lima bean stage.		bean embryo Ce	WBls:0000016
	At 20 Centigrade: 0-24 hours after L4-adult molt.		newly molted young adult hermaphrodite Ce	WBls:0000063