WormMine: AnatomyTerm WBbt:0004641

• Definition: Ventral cord motor neuron, innervates ventral body muscles
• Name: VB2
• Primary Identifier: WBbt:0004641
• Synonym: lineage name: W.aap

1 Children

Definition	Name	Synonym	Primary Identifier
nucleus of pedigree W.aap	W.aap nucleus		WBbt:0003219

5 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Single-cell RNA-Seq cell group 6_1 expressed in neuron.	scVI 0.6.0	WBPaper00065841:6_1
	Transcripts enriched in VB2 according to single cell RNAseq.	Genes that pass the Bonferroni threshold for multiple comparisons (q < 0.05) are significantly enriched.	WBPaper00066550:VB2_enriched
	Single-cell RNA-Seq cell group 6_0 expressed in neuron.	scVI 0.6.0	WBPaper00065841:6_0
	Transcripts enriched in VB02 according to single cell RNAseq.	Genes that pass the Bonferroni threshold for multiple comparisons (q < 0.05) are significantly enriched.	WBPaper00061651:VB02_enriched
	Transcripts enriched in VB according to single cell RNAseq.	Genes that pass the Bonferroni threshold for multiple comparisons (q < 0.05) are significantly enriched.	WBPaper00061651:VB_enriched

36 Expression Patterns

• Primary Identifier: Expr4784
• Pattern: Three independent transgenic lines display GFP fluorescence exclusively in VB motor neurons in the ventral nerve cord. ceh-12::GFP, is expressed in all 11 VB motor neurons and in a single head neuron, RID; occasional weak GFP expression was observed in the pharyngeal/intestinal valve cell and in the excretory gland cell.

• Primary Identifier: Expr15649

• Primary Identifier: Expr15558

• Primary Identifier: Expr15567

• Primary Identifier: Expr15571

• Primary Identifier: Expr15572

• Primary Identifier: Expr15573

• Primary Identifier: Expr15579

• Primary Identifier: Expr15586

• Primary Identifier: Expr15651

• Primary Identifier: Expr15652

• Primary Identifier: Expr15589

• Primary Identifier: Expr15591

• Primary Identifier: Expr15598

• Primary Identifier: Expr15604

• Primary Identifier: Expr14590
• Pattern: Embryonic expression of exc-7 was first observed at the bean stage. By reverse lineaging with use of SIMI-Biocell software, we confirm the identity of one of the expressing cells at this stage as the excretory canal cell. In L1 animals, broad expression in the head, ventral nerve cord (VNC), and tail was observed. In young adults, expression is notably observed in vulva cells. In the nervous system specifically, expression is observed in many neurons throughout the body, but unlike Drosophila Elav, exc-7::gfp it is not panneuronally expressed. We confirmed previously reported expression in cholinergic VNC MNs, but absence of GABAergic VNC MNs, consistent with previous reports (Fujita et al., 1999; Loria et al., 2003) and consistent with exc-7 functioning in cholinergic, but not GABAergic neurons to control alternative splicing (Norris et al., 2014). exc-7::gfp is also expressed in some non-neuronal cell types, including muscle and hypodermis, but not in the gut. A previous report showed that exc-7 is only transiently and weakly expressed in the excretory cell, which, based on exc-7's excretory mutant phenotype, has puzzled researchers (Fujita et al., 2003). We find that the gfp tagged exc-7 locus is strongly and continuously expressed in the excretory canal cell.

• Primary Identifier: Expr15608

• Primary Identifier: Expr15611

• Primary Identifier: Expr15570

• Primary Identifier: Expr521
• Remark: Transgenic Marker: rol-6(su1006).
• Pattern: Expression in touch-sensitive neurons, motor neurons, and interneurons which starts in the 2-fold embryo and disappears by L3. Sporadic expression seen in retrovesicular ganglion at L1. Expressed in ALM, PLM and BDU cells at 2-fold embryo, L1 and L2. In AVM, PVM cells at L1 and L2. Expressed in ventral cord motor neurons VA2-VA12, VB1-VB11, AVF cells, and precursors at L1 and L2.

• Primary Identifier: Expr15644

• Primary Identifier: Expr15646

• Primary Identifier: Expr12716

• Primary Identifier: Expr12717

• Primary Identifier: Expr15315

• Primary Identifier: Expr15316
• Pattern: acr-5 ORF

• Primary Identifier: Expr15633

• Primary Identifier: Expr12715

• Primary Identifier: Expr15314

• Primary Identifier: Expr626
• Remark: Reporter gene fusion type not specified. This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope).
• Pattern: First detected prior to hatching, staying constant in most cells throughout the larval and adult stages. Expression seen predominantly in neurons, ventral nerve cord, DA, DB, VA, and VB class-set throughout the entire length of the cord and several pharyngeal neurons. Cytoplasmic expression is also seen in the dorsal cord, in the processes of DA3-7, DB1-7, PDB. Also in pair of unidentified neurons of lateral ganglia. In the male tail, expression is detected in the DVA neuron and the glial cells (Balpha.1/rd and Bgammapl/r)that form the socket for the spicule starting at the L4 stage.

0 Life Stages

3 Parents

Definition	Name	Synonym	Primary Identifier
Neuron class of eleven motoneurons, distributed along the ventral cord, which innervate ventral body muscles.	VB neuron		WBbt:0005336
neuron whose cell body is in the retrovesicular ganglion.	retrovesicular ganglion neuron	retrovesicular ganglion	WBbt:0005403
post-embryonic cell of pedigree W.aa	W.aa		WBbt:0007569