WormMine: ExpressionPattern Expr4240

• Pattern: Very weak vulval muscle cell expression was observed for the F54C9.11::gfp fusion generated by fosmid recombineering. The GFP could just be seen despite the strong, background, endogenous, intestinal autofluorescence that appears yellow with the filter set used. CFP could not be detected for the F54C9.11::cfp fusion, but the filter set used to observe this fluor meant that the autofluorescence appeared blue, the same colour as the reporter signal, and thus a weak vulval expression may not have been discernable.
• Primary Identifier: Expr4240
• Remark: Curiously, when examining the worms for CFP fluorescence, a strong fluorescent signal was observed from lateral hypodermal nuclei. However, this was also seen in wild-type C.elegans and has nothing to do with the CFP reporter. With very careful observation this autofluorescence could again be seen, exceedingly weakly, with the GFP filter set but not with a variety of other filter sets that were available. This autofluorescence precludes claiming that no hypodermal expression was generated for the F54C9.11 fluorescent protein reporter fusions. Nevertheless, it would appear that F54C9.11 is expressed weakly in the vulval muscle cells and the hypodermal expression previously observed was indeed due to activation by regulatory elements of the co-transformation marker. Furthermore, the additional flanking DNA present for reporter gene fusions generated by fosmid recombineering may well insulate the fusion gene from such artifacts.

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
muscle associated with hermaphrodite vulva.	vulval muscle		WBbt:0005821

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00010045	rabi-1	F54C9.11	Caenorhabditis elegans

0 Life Stages