WormMine: AnatomyTerm WBbt:0004385

• Definition: Neuron class of one motor neuron, process in dorsal cord, cell body in pre-anal ganglion.
• Name: PDB
• Primary Identifier: WBbt:0004385
• Synonym: lineage name: P12.apa

1 Children

Definition	Name	Synonym	Primary Identifier
nucleus of pedigree P12.apa	P12.apa nucleus		WBbt:0002597

2 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Transcripts enriched in PDB according to single cell RNAseq.	Genes that pass the Bonferroni threshold for multiple comparisons (q < 0.05) are significantly enriched.	WBPaper00061651:PDB_enriched
	Single-cell RNA-Seq cell group 14_2 expressed in neuron.	scVI 0.6.0	WBPaper00065841:14_2

35 Expression Patterns

• Primary Identifier: Expr15649

• Primary Identifier: Marker49
• Remark: Picture: N.A. Reporter gene fusion type not specified.
• Pattern: Expressed in anterior neurons, including AIY, AIZ, RID, M5, ASI, and labial sensory neurons, VNC motorneurons, midbody neurons HSN, CAN, and PVM, tail neurons DVB, DVC, and PDB, and the nonneuronal excretory cell, uterine muscles. -- according to pers. comm. from Oliver Hobert.

• Primary Identifier: Expr15442

• Primary Identifier: Expr15558

• Primary Identifier: Expr15567

• Primary Identifier: Expr15571

• Primary Identifier: Expr15572

• Primary Identifier: Expr15573

• Primary Identifier: Expr15579

• Primary Identifier: Expr15586

• Primary Identifier: Expr15651

• Primary Identifier: Expr15652

• Primary Identifier: Expr15589

• Primary Identifier: Expr15591

• Primary Identifier: Expr15598

• Primary Identifier: Expr15604

• Primary Identifier: Expr14590
• Pattern: Embryonic expression of exc-7 was first observed at the bean stage. By reverse lineaging with use of SIMI-Biocell software, we confirm the identity of one of the expressing cells at this stage as the excretory canal cell. In L1 animals, broad expression in the head, ventral nerve cord (VNC), and tail was observed. In young adults, expression is notably observed in vulva cells. In the nervous system specifically, expression is observed in many neurons throughout the body, but unlike Drosophila Elav, exc-7::gfp it is not panneuronally expressed. We confirmed previously reported expression in cholinergic VNC MNs, but absence of GABAergic VNC MNs, consistent with previous reports (Fujita et al., 1999; Loria et al., 2003) and consistent with exc-7 functioning in cholinergic, but not GABAergic neurons to control alternative splicing (Norris et al., 2014). exc-7::gfp is also expressed in some non-neuronal cell types, including muscle and hypodermis, but not in the gut. A previous report showed that exc-7 is only transiently and weakly expressed in the excretory cell, which, based on exc-7's excretory mutant phenotype, has puzzled researchers (Fujita et al., 2003). We find that the gfp tagged exc-7 locus is strongly and continuously expressed in the excretory canal cell.

• Primary Identifier: Expr15608

• Primary Identifier: Expr15611

• Primary Identifier: Expr15570

• Primary Identifier: Expr8694
• Remark: Picture: Fig 3.
• Pattern: Expression in the alimentary canal: Strong and consistent expression in M5, I1, I3, I6, NSM. Weak or rare expression in posterior arcades. Expression in the nervous system: Phsh, ADA, ADE, ADL, AIN, AIY, ALM, AUA, AVA, AVD, AVH, AVJ, AVK, AVM, AWB, BDU, CAN, CEP, DAn, DBn, DDn, DVB, DVC, FLP, HSN, IL1, IL2, LUA, OLL, PDA, PDB, PDE, PHA, PHB, PHC, PLM, PLN, PVC, PVD, PVM, PVN, PVP, PVQ, PVR, PVT, PVW, RIB, RIC, RIF, RIP, RIS, RME, SDQ, SIA (early larva), SIB (early larva), SMB (early larva), SMD (early larva), URA, URB, VAn, VBn, VCn, VDn, M5, I1, I6, NSM. Expression in the reproductive system: In adult stage, expressed in vulval muscle, uterine muscle, HSN, VCn. In developing larva stage, expressed in HSN, VCn, and anchor cell.

• Primary Identifier: Expr15644

• Primary Identifier: Expr12716

• Primary Identifier: Expr12717

• Primary Identifier: Expr14654
• Pattern: The male-specific neuronal expression of lin-29a is not observed in male-specific neurons but is, interestingly, entirely restricted to sex-shared neurons (i.e. neurons that are generated in both sexes). Intriguingly, most of these neurons had not been previously reported to show sexually dimorphic gene expression patterns or functions. Specifically, 21 out of 116 sex-shared neuron classes express lin-29a, including seven sensory neuron classes in the head and tail (AWA, ASG, ASJ, ASK, ADF, PHB, PLM), seven interneuron classes (AVA, RIA, AIM, AVG, RIF, PVC, PVN), a few head and tail motor neurons (SAB, PDA, PDB) and most of the sex-shared ventral nerve cord motor neurons (dorsal and ventral A, B and D-type and AS neurons). Expression of lin-29a in all these neurons in the male nervous system is precisely temporally controlled; it is first observed in the early L4 stage and persists throughout adulthood.

• Primary Identifier: Expr1615
• Remark: Reporter gene fusion type not specified.
• Reporter Gene: pR13A14lacZ expression pattern were also characterized in a strain in which the reporter construct was stably integrated into the genome (ZB167), which gave results consistent with those observed for mosaic animals. An identical expression profile was observed when a second fusion, including 2.5 kb upstream of the predicted initiation codon, was assayed.
• Pattern: pR13A14lacZ activity is apparent in the embryo and is first detected during the 3-fold stage, about 300 min prior to hatching. This staining is likely to occur in the embryonically derived DA, DB, and/or DD motor neurons. Beta-galactosidase activity is observed in additional neurons later in development and peaks during the L2 stage when intense staining of sensory neurons, interneurons in the nerve ring, and motor neurons in the ventral nerve cord are apparent. Strong staining persists into adulthood. Among the neurons that express pR13A14lacZ are the ASH and FLP sensory neurons, the PVM touch neuron, the PVC, AVB, AVA, and AVD command interneurons, and the PDA, PDB, DA, DB, DD, VA, VB, and VD classes of motor neurons. Additional neurons situated either within or in the vicinity of the nerve ring express the R13A1_4 reporter gene. In summary, R13A1_4 expression is neuron specific and restricted to about 45 cells.

• Primary Identifier: Expr12722

• Primary Identifier: Expr1880
• Pattern: Expression of the reporter is first detected in embryos at around the 50-cell stage in 23 cells and widens during embryonic development. At the comma stage, expression is seen in a set of cells whose position is consistent with neuroblasts in the tail as well as in the head where they form a ring-like structure. In larval stages and throughout adult stages, expression is largely restricted to a set of neurons in several head ganglia (AIY, AIZ, RID, M5, ASI, and subsets of labial sensory neurons), motor neurons in the ventral nerve cord, neurons in the midbody region (HSN, CAN, and PVM) and in the tail ganglia (DVB, DVC, and PDB). Consistent nonneuronal expression could be observed in the excretory cell and uterine cells.

• Primary Identifier: Expr15162
• Pattern: Expression of gnrr-6 localized to SIA sublateral motor neurons and AVB forward command interneurons, which is in agreement with single-cell RNA-Seq data. In addition, we observed expression in PDB and PHC neurons in the tail and few sensory neurons in the head.

• Primary Identifier: Expr15620

0 Life Stages

3 Parents

Definition	Name	Synonym	Primary Identifier
neuron making synapse on an effector (muscle, gland).	motor neuron	motoneuron	WBbt:0005409
neuron with cell body in the preanal ganglion.	preanal ganglion neuron	preanal ganglion neurons	WBbt:0005447
post-embryonic cell of pedigree P12.ap	P12.ap		WBbt:0007233