WormMine: ExpressionPattern Expr9390

• Pattern: The LTD-1::GFP signal is present throughout the development of C. elegans. This signal was detected as early as the 2-fold embryo. The ltd-1 reporter is also expressed throughout the seam cell division process. Its expression is observed in the seam cells of the early embryo and in the larval stages once these cells have commenced division. The LTD-1::GFP signal is also observed in rectal epithelial cells (tentatively, U and Y cells) and in the terminal bulb (marginal cells) and isthmus of the pharynx from hatching to adulthood.
• Primary Identifier: Expr9390
• Subcellular Localization: Sub-cellular localization within the body wall muscle: Dense bodies, Cytoplasm, +/- Nucleus The LTD-1::GFP construct is expressed in the apical regions of the dorsal and ventral hypodermis in very tightly organized circumferential filament bundles. This cytoskeletal expression pattern mirrors the intracellular distribution of actin and tubulin in C. elegans. In late embryos, the GFP signal is localized to the apical junction between hyp 5, hyp 6 and hyp 7 and between the seam cells and the P blast cells in the ventral midline. This signal highlights the cell fusion processes that take place during post-embryonic development. The ltd-1 reporter is also expressed throughout the seam cell division process. It clearly outlines the cytokinesis between posterior mother cells and anterior daughter cells and illustrates the subsequent fusion of the anterior daughter cells to the hypodermal syncitium. The GFP signal is also observed in longitudinal filaments within the cytoplasm linking both extremities of the elongating seam cells and in the alae formed by their fusion.

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
a section of pharynx of which muscles contract with a peristaltic motion.	isthmus		WBbt:0003734
a type of pharyngeal cell that connects basement membrane to the apices of triangle-shaped pharyngeal lumen.	marginal cell		WBbt:0003673
These cells include: B, F, Y, U, K' and K. All rectal epithelial cells contain secretory membrane stacks along this region facing the lumenal cuticle and produce some portion of the cuticular lining of the rectum. These are interfacial cells that connect the alimentary system to the main body syncytium.	rectal epithelial cell	rectal epithelium	WBbt:0008182

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003089	ltd-1	K02C4.4	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023