WormMine: ExpressionPattern Expr99

• Pattern: Expression is first seen in late embryogenesis and extends through to adulthood. There are two components to this strongly expressing pattern. The first component involves the nucleus and canals of the excretory cell. The excretory cell is first seen to express b-galactosidase in early 3-fold embryos. b-galactosidase activity in the excretory canals is detectable soon after, with older embryos showing expression extending anteriorly and posteriorly through the canals. Expression in the posterior canals is present through to adulthood, with staining extending posteriorly along 3/4 the length of the animal. The anterior component of the excretory canal begins to fade around the L2 stage when the 2nd component begins to express. Staining in the anterior canals of the excretory cell is generally only faint and diffuse in older larvae and adults, while that in the posterior arms remains quite strong.The expression seen in this component is consistent with the gene product's predicted function as a sodium/dicarboxylate symporter. The second component involves three symmetric processes that start from the anterior of the terminal bulb and extend through the procorpus of the pharynx. Expression is seen from L2 stage onwards. The pattern appears as strong staining in the subcellular foci of these processes. The expression appears to become weaker immediately posterior to the metacorpus, and then intensifies over the metacorpus. It fades out near the extreme anterior of the procorpus. There is some mosaicism in this component as not all parts express in 100% of worms. The most common expression involves all 3 parts in the region of the isthmus and only 1 or 2 parts expressing in the procorpus. This expression corresponds to the marginal cells of the pharynx. The same expression is seen in all injected lines. Expression has not been examined in males.
• Primary Identifier: Expr99
• Remark: Other strain-- UL420 late embryo(author) = 3-fold embryo + fully-elongated embryo(curator).

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
H-shaped cell associated with the excretory system, largest cell in C. elegans.	excretory cell	excretory canal cell	WBbt:0005812
a type of pharyngeal cell that connects basement membrane to the apices of triangle-shaped pharyngeal lumen.	marginal cell		WBbt:0003673

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001624	glt-6	R05G6.6	Caenorhabditis elegans

7 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020