WormMine: ExpressionPattern Expr1625

• Pattern: Maternally expressed DIE-1 can be detected in all blastomeres of the early embryo. Zygotic DIE-1 expression is prominent in the posterior half of the embryo, in the nuclei of muscle and hypodermal precursors. Shortly after the hypodermal cells undergo their terminal divisions, DIE-1 can be detected very strongly in the dorsal hypodermis. As intercalation proceeds, DIE-1 expression becomes weaker in these cells. The "pointer" cells, which are the last dorsal hypodermal cells to intercalate, maintain elevated levels of DIE-1 longer than other dorsal hypodermal cells. DIE-1 is undetectable in the nuclei of C-derived dorsal cells by the time intercalation is complete. In addition to expression in dorsal hypodermal cells, DIE-1 is present in ventral hypodermal cells during the process of enclosure; expression ceases in the hypodermis after enclosure has completed, prior to the process of elongation. DIE-1 is also present in pharyngeal cells and in the gut primordium. DIE-1 is also expressed in muscle cells, as confirmed by double-staining die-1::gfp-expressing embryos with anti-GFP and muscle-specific antibodies. Nuclei containing GFP indicative of DIE-1 expression were consistently observed within the rows of muscle. After enclosure is complete, DIE-1 expression completely disappears in all tissues until late in embryogenesis, at which time ~15 cells between the anterior and posterior bulbs of the pharynx express die-1::gfp. DIE-1 is also present in numerous cells along the ventral midline in L2L4 larvae.
• Primary Identifier: Expr1625
• Remark: Reporter gene expression pattern confirmed to match exactly with antibody staining.
• Reporter Gene: Expression pattern was observed with anti-GFP rabbit antibody staining.
• Subcellular Localization: nuclei

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
Contractile cell that makes up muscle tissue.	muscle cell		WBbt:0003675

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000995	die-1	C18D1.1	Caenorhabditis elegans

4 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003