WormMine: ExpressionPattern Expr9090

• Pattern: A rescuing madd-2::gfp transgene was expressed in most neurons, in hypodermis, and in muscle cells. In many neurons, madd-2::gfp expression was transient, peaking during periods of axon outgrowth and guidance. madd-2::gfp was most widely and strongly expressed in the embryo, particularly during the initial period of axon outgrowth at 350 to 400 min of development. At this stage, madd-2::gfp was prominent in the anterior embryo, including the developing neurons of the nerve ring, in the developing motor neurons of the ventral nerve cord, in posterior neurons, and in hypodermal cells. At ~430 min of development (1.5-fold stage), the madd-2::gfp transgene exhibited a more restricted pattern of expression in the anterior, ventral, and posterior regions of the embryo. The extinction of reporter gene expression in many neurons coincides with the period when most axons in the nerve ring and ventral cord have reached their targets. At the first larval stage, continued high expression of the madd-2::gfp transgene was observed in some head neurons, head muscles, ventral motor neurons, neurons in the tail, and hypodermal cells. As the animals developed from larvae into adults, madd-2::gfp expression decreased substantially in head and tail neurons. madd-2::gfp expression was detected in the HSN motor neurons around the third larval stage, during the period of its ventral axon outgrowth. Although reporter gene fusions should be interpreted with caution, these results suggest that MADD-2 could be present in many Netrin-responsive neurons at the time of axon guidance.
• Primary Identifier: Expr9090
• Remark: Picture: Figure 4.
• Subcellular Localization: In most cells, MADD-2::GFP protein was in irregular puncta in the cytoplasm.

5 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
Major cell type of nervous tissue, specialized for transmission of information in the form of patterns of impulses.	neuron	neurone	WBbt:0003679
Contractile cell that makes up muscle tissue.	muscle cell		WBbt:0003675
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNL	lineage name: ABplapppappa	WBbt:0004758
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNR	lineage name: ABprapppappa	WBbt:0004757

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00016539	madd-2	C39F7.2	Caenorhabditis elegans

7 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015