WormMine: ExpressionPattern Expr2747

• Pattern: In situ immunohistochemistry with this antiserum shows that GPB-1 is present in early embryos. Diffuse staining can be seen at the 1-cell stage. From the 2-cell stage onward. Up to the beginning of morphogenesis, staining continues at approximately equal levels in most or all cells. Once tissue differentiation occurs, staining is brightest in neuronal cells. In larval and adult stages, GPB-1 expression is seen in most or all neurons, including the nerve ring, the dorsal and ventral nerve cords, and the preanal ganglion. In addition, the somatic gonad, vulva, and hypodermal seam cells have high expression. Other tissues, such as the intestine, pharynx, and body wall muscles, appear to have a low level of expression. GPB-1 is also expressed in the germline.
• Primary Identifier: Expr2747
• Remark: The specificity of the antibody staining is indicated by the following controls: first, the staining is competed away by preincubation of the antibody with GPB-1 protein and, second, gpb-1 mutant embryos do not exhibit any staining.
• Subcellular Localization: GPB-1 is detected most strongly at the cell membrane, with staining concentrated at the contact between cells. Interestingly, staining also colocalizes with the asters (arrays of microtubules emanating from the centrosomes) just before and during early cell divisions.

14 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
Major cell type of nervous tissue, specialized for transmission of information in the form of patterns of impulses.	neuron	neurone	WBbt:0003679
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
adult vulval toroid cell, F, formed by fusion of precursors of lineage names P6.papl, P6.papr, P6.ppal and P6.ppar.	vulF		WBbt:0006768
adult vulval toroid cell, E, formed by fusion of precursors of lineage names P6.paal, P6.paar, P6.pppl and P6.pppr.	vulE		WBbt:0006767
organ producing either sperm or ova.	gonad		WBbt:0005175
adult vulval toroid cell, C, formed by fusion of precursors of lineage names P5.ppal, P5.ppar, P7.papl and P7.papr.	vulC		WBbt:0006765
adult vulval toroid cell, D, formed by fusion of precursors of lineage names P5.ppp and P7.paa.	vulD		WBbt:0006766
adult vulval toroid cell, B2.	vulB2		WBbt:0006764
adult vulval toroid cell, B1, formed by fusion of precursors of lineage names P5.papa and P7.ppap.	vulB1		WBbt:0006763
adult vulval toroid cell, A, formed by fusion of precursors of lineage names P5.paaa, P5.paap, P7.pppa and P7.pppp	vulA		WBbt:0006762

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001679	gpb-1	F13D12.7	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003