WormMine: ExpressionPattern Expr9444

• Pattern: nlg-1 is expressed in a subset of neurons in C. elegans adults, including ~20 cells in the ventral nerve cord and ~20 cells in the head. The nlg-1-expressing cells in the ventral nerve cord were identified as the cholinergic VA and DA motor neurons. Authors also identified the two AIY and two URB interneurons and the four URA motor neurons in the head, and the two PVD mechanosensory and two HSN motor neurons in the body, as nlg-1-expressing cells. Finally, faint Pnlg-1::YFP expression was also observed in body wall muscles.
• Primary Identifier: Expr9444
• Subcellular Localization: Bright punctate staining was observed in dendritic (postsynaptic) regions. Clear punctate staining was also observed in presynaptic regions of each neuronal type. For example, in the DA9 motor neuron, bright NLG-1::YFP puncta were present in the ventral postsynaptic domain, and dimmer puncta were present in the dorsal presynaptic region. Puncta were excluded from the synapse-poor region between the cell body and dorsal presynaptic region and from the anterior asynaptic region of the dorsal process. To further study the punctate staining in the presynaptic region, NLG-1::YFP localization was examined in animals expressing the tagged synaptic vesicle protein mCherry::RAB-3 in DA9. In the dorsal axon, NLG-1::YFP puncta partially colocalized with puncta containing mCherry::RAB-3, suggesting that NLG-1::YFP localization is perisynaptic. Sub-cellular localization within the body wall muscle: Muscle cell membrane +/- Muscle arms

18 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring.	ventral nerve cord	ventral cord	WBbt:0005829
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
Neuron class of twelve motoneurons, distributed along the ventral cord, which innervate ventral body muscles.	VA neuron		WBbt:0005339
Neuron class of nine ventral cord motor neuron, innervates dorsal muscle.	DA neuron		WBbt:0005278
Major cell type of nervous tissue, specialized for transmission of information in the form of patterns of impulses.	neuron	neurone	WBbt:0003679
Amphid interneuron	AIYL	lineage name: ABplpapaaap	WBbt:0003963
Amphid interneuron	AIYR	lineage name: ABprpapaaap	WBbt:0003961
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNL	lineage name: ABplapppappa	WBbt:0004758
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNR	lineage name: ABprapppappa	WBbt:0004757
anterior-most body region containing the pharynx.	head		WBbt:0005739
Neuron, lateral process adjacent to excretory canal	PVDR	lineage name: V5R.paapa	WBbt:0004088
Neuron, lateral process adjacent to excretory canal	PVDL	lineage name: V5L.paapa	WBbt:0004090
Ring motor neuron	URAVL	lineage name: ABplpaaapaa	WBbt:0004935
Ring motor neuron	URAVR	lineage name: ABprpaaapaa	WBbt:0004934
Neuron, presynaptic in ring, ending in head	URBL	lineage name: ABplaapaapa	WBbt:0004932
Neuron, presynaptic in ring, ending in head	URBR	lineage name: ABpraapaapa	WBbt:0004930
Ring motor neuron	URADL	lineage name: ABplaaaaaaa	WBbt:0004940
Ring motor neuron	URADR	lineage name: ABarpapaaaa	WBbt:0004936

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006412	nlg-1	C40C9.5	Caenorhabditis elegans

0 Life Stages