WormMine: ExpressionPattern Expr2874

• Pattern: The transgenic animals started to express the construct during late embryogenesis. GFP expression was not found in early embryogenesis. A late embryo showed diffuse expression. In early larvae, a faint and diffuse staining was observed in all tissues. The C05D11.3::GFP continued to be expressed in most cells of the nervous system, at all larval stages and in adult animals: the ventral nerve cord as well as dorsal and lateral cords. And it is expressed in the pharynx. Larval stages showed strong expression, in particular non-neuronal cells. Furthermore, an oval of fluorescence in the middle of the L4 worm suggested that C05D11.3 was expressed in the vulva. After the L4 stage, C05D11.3::GFP expression was seen in the vulva in all life. A more detailed analysis of this expression indicate that C05D11.3 is highly expressed in the nervous system and vulva.
• Primary Identifier: Expr2874
• Subcellular Localization: C05D11.3::GFP is partly co-localized with DAPI, suggesting that C05D11.3 is localized both in the nucleus and the cytoplasm.

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
female genital.	vulva		WBbt:0006748
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
Complement of nervous tissue (neurones, nerves, receptors and support cells) serving to detect, relay and coordinate information about an animal`s internal and external environments and to initiate and integrate its effector responses and activities.	nervous system		WBbt:0005735

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006515	txdc-9	C05D11.3	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021