WormMine: ExpressionPattern Expr3238

• Pattern: GFP-RME-6 was expressed in many tissues, including coelomocytes, oocytes, hypodermis, intestinal and pharyngeal cells.
• Primary Identifier: Expr3238
• Reporter Gene: Transgenic worms that expressed RME-6 with either N- or C-terminal GFP tags, each under the control of the rme-6 promoter. Expression of these RME-6 fusion proteins fully rescues the coelomocyte endocytosis defect of rme-6(b1014), indicating that both GFP fusions are functional and are very likely to be correctly localized.
• Subcellular Localization: In intestinal and pharyngeal cells, GFP-RME-6 was clearly concentrated on the plasma membrane. In coelomocytes, GFP-RME-6 was also localized on or very close to the plasma membrane. Most GFP-RME-6 did not overlap with mRFP1-EEA-1 or mRFP1-RAB-5. In contrast, strong colocalization of GFP-RME-6 was found with mRFP1-CHC-1 and mRFP1-alpha-adaptin on or near the plasma membrane of coelomocytes. mRFP1-CHC-1 and mRFP1-alpha-adaptin as well as GFP-RME-6 were distributed unevenly around the plasma membrane, with regions of high and low labelling, reminiscent of the uneven distribution of endocytic pit structures that can be seen in coleomocytes by electron microscopy. Similar colocalization was observed with mRFP-RME-6 and GFP-CHC-1. These results indicate that most RME-6 is associated with clathrin-coated pits and that only a minor fraction of the RME-6 in the cells is associated with early endosomes. Similar subcellular localization of GFP-RME-6 was observed in oocytes and the hypodermis. To analyse RME-6 distribution in oocytes, strains that express GFP-RME-6 in the rme-6(b1014) background under pie-1 promoter control were established . Expression of GFP-RME-6 in oocytes completely rescued the yolk endocytosis defect of the rme-6(b1014)-null mutant. GFP-RME-6 was concentrated on small puncta, which were on or close to the plasma membrane of oocytes. Strong colocalization of this GFP-RME-6 with mRFP1CHC-1 surface puncta was observed, indicating that RME-6 labels coated pits or vesicles. On the other hand, GFP-RME-6 in oocytes showed little spatial overlap with endogenous EEA-1, as assayed using a previously published anti-EEA-1 antibody. Clear colocalization of GFP-RME-6 and mRFP-CHC-1 was also evident in hypodermal cells. RME-6 and coated pit marker colocalization was not perfect and that the ratio of intensities of GFP-RME-6 and mRFP1-CHC-1 varied from puncta to puncta, implying that GFP-RME-6 may associate with clathrin-coated pits at a specific stage in their formation.

5 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
a female haploid germ cell.	oocyte	ovum	WBbt:0006797
A free-floating spherical cell lying in the pseudocoelomic cavity of larvae and adult C. elegans which can endocytose many compounds, possibly for immune surveillance. There are six coelomocytes in adult hermaphrodites, and they display prominent cytoplasmic inclusions and vacuoles.	coelomocyte		WBbt:0005751

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004377	rme-6	F49E7.1	Caenorhabditis elegans

0 Life Stages