WormMine: ExpressionPattern Expr3808

• Pattern: Not detected in embryos via immunostaining. Expressed in adult worms. Immuno-fluorescent staining of PFN-2 was strong in the intestinal wall, the spermatheca, and the pharynx.
• Primary Identifier: Expr3808
• Remark: Although anti-PFN-2 and anti-PFN-3 antibodies showed specific reactivity for respective isoform on Western blot of total C. elegans lysates, they had some reactivity to null mutants in immunofluorescent staining. Therefore, these antibodies were additionally adsorbed to acetone fixed powders of pfn-2 and pfn-3 null mutant worms respectively to ascertain the specificity of these antibodies. These treatments significantly reduced nonspecific staining of the respective null mutant. The patterns of expression and localization of PFN-2 in the pfn-3 null mutants were indistinguishable from those in wild type.

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003990	pfn-2	F35C8.6	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041