WormMine: ExpressionPattern Expr3952

• Pattern: In larvae and adult hermaphrodites, the expression of a Pdyn-1 dyn-1::gfp reporter was observed in all previously described dyn-1-expressing tissues, including gonadal sheath cells. In embryos, many cell types express Pdyn-1 dyn-1::gfp, including known engulfing cells such as the hypodermal cells, intestinal precursor cells, and the entire pharyngeal primordium.
• Primary Identifier: Expr3952
• Remark: Site of action: To determine whether DYN-1 activity is required in the engulfing cell or the dying cell for the removal of cell corpses, dyn-1 cDNA was expressed under the control of the engulfing cell-specific promoter Pced-1 or the dying cell-specific promoter Pegl-1 and authors examined the ability of these transgenes to promote engulfment. Pced-1 dyn-1::gfp rescued the Ced phenotype of dyn-1(n4039) to the same extent as Pdyn-1 dyn-1cDNA. In contrast, Pegl-1 dyn-1::gfp did not exhibit significant rescuing activity, although it produced a GFP signal in dying cells. These results indicate that the dyn-1 activity in engulfing cells, but not in dying cells, is sufficient for the rescue of the Ced phenotype. dyn-1 thus primarily functions in engulfing cells for the removal of apoptotic cells.

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
five pairs of thin gonadal sheath cells form a single layer covering the germ line component of each arm, each pair occupying a stereotyped position along the gonad proximal-distal axis.	gonadal sheath cell		WBbt:0005828

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001130	dyn-1	C02C6.1	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024