WormMine: ExpressionPattern Expr8551

• Pattern: After becoming adult worm, pqbp-1.1-Venus expression reduced rapidly in pharynx, while the expression continued in intestinal cells. During the development, somatic gonad cells expressed pqbp-1.1-Venus only from L3 to L4 stage, and adult gonads or fertilized eggs did not express pqbp-1.1-Venus. Pqbp-1.1 expression was also observed in head ganglion cells at L1 stage. Some neurons in the tail show weak expression of pqbp-1.1-Venus in adult worms. However, after L1 stage, expression in neurons was relatively weak in comparison to that in intestinal cells or somatic gonad, and further analysis is needed to clarify the detail. pqbp-1.1 was expressed from 24-cells stage to Larva stage 4 (L4). From 24 cells- to gastrulation-stage, pqbp-1.1-Venus was expressed ubiquitously. At L1 stage, neural cells in the head ganglion express the fusion protein. From late L1 stage, intestinal cells expressed pqbp-1.1-Venus strongly and the high expression continued to L4 stage. The peak of expression was observed at L3 stage. Pharyngeal cells show an almost similar pattern of expression during development, while the peak seems to be at L4 stage.
• Primary Identifier: Expr8551
• Remark: Picture: Figure 2, 3, 4.
• Subcellular Localization: In pharynx, higher magnification of the signals revealed that the fusion protein formed speckle-like intranuclear dots (or nuclear bodies) just like mammalian PQBP1.

5 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
neuron with its cell body situated in the tail, posterior to rectum.	tail neuron		WBbt:0006759
organ producing either sperm or ova.	gonad		WBbt:0005175

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00020647	pqbp-1.1	T21D12.3	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003