WormMine: ExpressionPattern Expr9049

• Pattern: In the absence of metal, the cellular pattern of NUMR-1 expression was identical to that observed in JF85(mtEx60) nematodes . NUMR-1 was constitutively expressed in intestinal cells of L1 nematodes and the level of expression decreased as the nematodes developed. NUMR-1 was also expressed in neurons in the head, egg-laying muscles of the vulva, and a few cells in the tail. When exposed to cadmium, NUMR-1 expression increased in intestinal and pharyngeal nuclei. This pattern was similar to that observed for JF85(mtEx60) C. elegans. Following copper exposure, NUMR-1 expression in adult nematodes increased only in pharyngeal nuclei. Similar patterns of expression were observed in larval C. elegans in the presence and absence of the metals.
• Primary Identifier: Expr9049
• Remark: Picture: Fig 4, Fig 5.
• Subcellular Localization: NUMR-1 was concentrated in the nuclei of the expressing cells. Nuclear-accumulated NUMR-1GFP was clearly observed as punctate structures within the intestinal nuclei of cadmium-treated nematodes. NUMR-1 colocalized with HSF-1 after cadmium treatment. This result suggests that NUMR-1 may be a component of nuclear stress granules.

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
muscle associated with hermaphrodite vulva.	vulval muscle		WBbt:0005821
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00017270	numr-1	F08F8.5	Caenorhabditis elegans

0 Life Stages