WormMine: ExpressionPattern Expr13308

• Pattern: The DAF-28::mCherry expression followed the reported expression pattern of pdaf-28::GFP transcriptional reporter, with fluorescent protein detected in head and tail neurons, pharynx, hypodermis, and other tissues. DAF-28::mCherry was efficiently secreted upon expression in L1 stage, as detected by its uptake by endocytic scavenger cells, coelomocytes. Interestingly, compared to the DAF-28::mCherry fusion protein, the previously described DAF-28::GFP protein was not efficiently secreted, as judged by coelomocyte fluorescence, and instead appeared to accumulate in neuronal cell bodies. This may be due to misfolding of the GFP moiety.
• Primary Identifier: Expr13308
• Subcellular Localization: Confocal imaging showed that DAF-28::mCherry protein is predominantly found in a punctate pattern reminiscent of the secretory vesicles in mammalian cells expressing insulin or IGF, with some puncta found in a regularly spaced pattern adjacent to the ASI axons. DAF-28::mCherry was efficiently secreted upon expression in L1 stage, as detected by its uptake by endocytic scavenger cells, coelomocytes.

5 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
neuron with its cell body situated in the tail, posterior to rectum.	tail neuron		WBbt:0006759
A free-floating spherical cell lying in the pseudocoelomic cavity of larvae and adult C. elegans which can endocytose many compounds, possibly for immune surveillance. There are six coelomocytes in adult hermaphrodites, and they display prominent cytoplasmic inclusions and vacuoles.	coelomocyte		WBbt:0005751

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000920	daf-28	Y116F11B.1	Caenorhabditis elegans

0 Life Stages