WormMine: ExpressionPattern Expr1347

• Pattern: RME-1 was most abundant in the germline, intestine, pharynx and coelomocytes.
• Primary Identifier: Expr1347
• Subcellular Localization: Throughout larval and adult stages, anti-RME-1 antibodies detected RME-1 associated with membranes and RME-1 diffuse in the cytoplasm of all cells. In germ cells, RME-1 was strongly concentrated in punctate structures underneath the plasma membrane. RME-1 was also present in the cytoplasm of germ cells, but was not found in nuclei. This was true for immature germ cells and for fully grown oocytes. Cytoplasmic RME-1 in intestinal cells was less diffuse than in germ cells. RME-1 was observed in the cytoplasm of intestinal cells in three different localizations: in small punctate structures (probably vesicles), in tubulo-vesicular structures near the basal (but not apical) plasma membrane, and in rings around the periphery of large vesicular structures, deeper within the cytoplasm. In some preparations, the rings appeared smooth, and were variegated with regions of high and low labelling in other preparations. Further experiments indicated that some of these structures, if not all, are endosomes. RME-1 is a peripheral endosomal protein in the intestine, and is localized in structures arising from basolateral endocytosis.

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784
A free-floating spherical cell lying in the pseudocoelomic cavity of larvae and adult C. elegans which can endocytose many compounds, possibly for immune surveillance. There are six coelomocytes in adult hermaphrodites, and they display prominent cytoplasmic inclusions and vacuoles.	coelomocyte		WBbt:0005751

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004373	rme-1	W06H8.1	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	All C. elegans development stages, including embryo, larva and adult stage.		all stages Ce	WBls:0000002