WormMine: ExpressionPattern Expr9878

• Pattern: The expression pattern of lin-3 in wild-type animals was determined at the late L2 to early L3 stage when vulval induction occurs. Robust expression of lin-3 was observed in the anchor cell and throughout the pharynx. Expression of lin-3 was also seen in the germline. In some wild-type animals a few copies of lin-3 mRNA were seen in one or more cells in the tail, on the ventral side slightly anterior to the anus. In addition, a few copies of lin-3 mRNA were seen on the ventral side of the animal, slightly behind the posterior gonad arm. We imaged several animals that were slightly older, in the late L3 stage, and observed expression of several copies of lin-3 mRNA in the region where P6.p and its descendants are located (data not shown). We did not consistently detect any lin-3 mRNA in other tissues, although in some animals we observed a single lin-3 mRNA molecule elsewhere (e.g. in or near an intestinal cell).
• Primary Identifier: Expr9878

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784
posterior region, from rectum to the end	tail		WBbt:0005741
anchor cell, induces vulva, part of hermaphrodite gonad.	anchor cell	AC	WBbt:0004522

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00002992	lin-3	F36H1.4	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027