WormMine: ExpressionPattern Expr7954

• Pattern: In adult worms, a K10C2.4:GFP fusion protein is expressed strongly in the intestine and the hypodermis.
• Primary Identifier: Expr7954
• Remark: Picture: Figure 2F.
• Reporter Gene: Cells expressing K10C2.4 were identified by photographing the ALF100 (bafIs100), ALF101 (bafIs101), and ALF102 (bafIs102) strains. These carry the WRM0615cC09 fosmid containing 34,131 bp of genomic DNA centered on the K10C2.4 gene. This fosmid was modified via recombineering to express an N-terminal GFP fusion protein and fitted with an unc-119 rescue fragment via cre-lox recombination. The modified fosmid was bombarded into DP38 (unc-119(ed3)) worms via microparticle bombardment.

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Epidermal layer.	hypodermis	epidermis	WBbt:0005733

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00019620	fah-1	K10C2.4	Caenorhabditis elegans

0 Life Stages