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Strain UL2626, UL2627 |
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Expr13606
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Larval and adult expression in the posterior intestine, body neurons, tail hypodermis, pseudocoelom and an unidentified structure around anus. Occasional spermathecae and excretory gland expression. Widespread expression in comma stage embryos. No intestinal, neuronal, hypodermal, excretory cell or pseudocoelom expression seen previously. No vulval expression seen in the current study. Larval and adult expression in the posterior intestine, body neurons, tail hypodermis, pseudocoelom and an unidentified structure around anus. Occasional spermathecae and excretory gland expression. Widespread expression in comma stage embryos. |
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Clone: pUL#JS10B7 |
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Expr7589
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Expression was seen in many tissues from the early embryo onwards. Tissues included nerves, excretory cell, coelomocytes, pseudocoelom, pharynx, seam cells, body wall muscle, vulva and spermatheca. |
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Clone: pUL#JS4E12 |
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Expr7588
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Expression is seen in many tissues, and may be ubiquitous. A high level of GFP expression is seen in the pharynx and in the intestine of all stages. There is also expression in the L4 and hermaphrodite spermathecae and vulva. Expression was seen in the pseudocoelom. |
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Picture: Figure 1, and S1. |
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Expr8115
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The expression pattern seen was the same in all eight lines. Strong expression of GFP was first detected in the embryo at the E16 stage in the intestine primordium. In later stage embryos, expression was also seen in other epithelial tissues. In larval worms, and throughout the adult stage, expression was seen in the intestine, pharynx, hypodermis, seam cells, spermatheca, and uterus. During postembryonic development, several nonepithelial cells also expressed the transgene, including many neurons in the nerve ring and coelomocytes, scavenger cells situated in the pseudocoelom. Within the nervous system, expression was not uniform but higher in a subset of neurons. |
At the subcellular level, in most cells the protein accumulated in punctate structures within the cytoplasm. Some protein was present very close to the plasma membrane and may be associated with it. In polarized epithelia, expression of the protein was predominantly on the baso-lateral parts of the cell and was largely absent from the apical cell membrane or the apical cytoplasm. In the intestine, seam cells, ventral hypodermis, pharynx, and other polarized epithelial cells, the most apical boundary of expression coincided with the adherens junctions that separate baso-lateral and apical cell membranes. Localization of the GFP fusion protein close to the junctions in the intestine and seam cells was observed. |
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Clone: pUL#IAH10C6 |
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Expr7428
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All 6 independent lines showed same expression, some lines more mosaic than others. Expression was primarily in mid-ventral body wall muscle cells of late larvae and adults. In old adults expression was also seen in the pseudocoelom. Ocassionally expression was seen in more posterior body wall muscle cells. Expression in anterior body wall muscle cell, which may be artefactual, was seen at different strengths in the different lines. |
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Clone: pUL#JS9B4 |
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Expr7640
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Expression is seen in nerves of the head and also faintly in the pseudocoelom. There is also some expression in the posterior gut. Expression is seen from the late embryo onwards. |
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Clone: pUL#IAH/AA4 |
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Expr7552
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2 of 3 lines gave strong expression throughout the intestine, late embryogenesis to adult. (Other line showed no expression.) UL2475 is possibly an integrated line. UL2466 gave mosaic expression. Some expression is seen in anterior muscle cells and some head nerve cells. Some globules of fluorescent protein seen in pseudocoelom, which may have escaped from the intestine. |
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The alr-1p::GFP reporter construct was generated by inserting 1 kb of upstream regulatory sequence into the pPD95.75 vector, which contains the green fluorescent protein (GFP) coding sequence and the 3' untranslated region of unc-54 at the 3' end. This construct was injected into N2 worms at 10 ng/ul along with a PCR product corresponding to 6 kb of overlapping alr-1 upstream regulatory sequence and a dominant Roller marker, pRF4 containing rol-6 (su1006),at 100 ng/ul to generate kuEx146. |
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Expr3525
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Embryonic analysis indicated an early expression pattern just after the 28-cell stage. The strongest expression at this point was seen in descendants of the C linage as well as less prominent expression within a subset of the AB lineage. By the comma stage (-400 cells), GFP expression was apparent in alternating dorsal hypodermal cells before the onset of cell fusions. After the onset of the hypodermal cell fusions, GFP was apparent throughout the hyp7 hypodermal syncytium. At the comma stage, GFP was also strongly expressed in the precursors to the PLM and ALN neurons, the T-cells (precursors to the phasmid socket cells), and the cells that would comprise the hyp4 anterior hypodermal syncytium. These cells were tentatively identified based on cell position and the strong expression seen within the adult structures derived from these cells. A number of GFP-expressing cells within the head region of the embryo remained unidentified. These cells likely include a number of head and pharyngeal neurons, although other cells types are not ruled out. Expression in the larval and adult hermaphrodite was primarily restricted to a subset of neurons and neuronal support cells. The PLM, ALM, and AVM touch neurons and the intrinsic pharyngeal neurons I2 and I6 all showed very strong expression throughout all larval stages and adulthood. The RIS neuron and one other unidentified, unpaired neuronal cell body located in the retrovesicular ganglion occasionally showed faint expression. Strong expression was also seen in the glial-like amphid and phasmid socket cells (AMso and PHso 1 and 2) throughout larval development and adulthood. Strong GFP expression was also observed within the hyp6 and hyp4 hypodermal syncytia, the distal most segments of the intestine and the coelomocytes, the scavenger cells located within the pseudocoelom. Variable GFP expression was also seen in larval and adult worms within the hyp7 hypodermal syncytium. Importantly, GFP expression was not observed in 23 of the 24 GABAergic neurons (the sole exception being the RIS neuron) that have been shown previously to stain with anti-ALR-1 antibodies (See Expr3487). This suggested that the sequences required for ALR-1 expression in these neurons may reside outside of the 6 kb of promoter sequence driving this GFP reporter. Alternatively, GFP expression from this construct may not be readily detectable above background fluorescence in these specific neurons. |
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Expr11071
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PGRN-1::RFP fusion protein was not found in discrete tissues. Instead, it was visible in what appeared to be intestinal organelles, in the pseudocoelomic cavity, and in scavenging coelomocytes, suggesting that, as in mammals, progranulin is secreted. |
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Expr12089
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Animals carrying the full-length ASP-6::GFP fusion show diffuse fluorescence in the pseudocoelom, indicating that the fluorescent chimera is secreted. |
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Expr12674
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VIT- 2::GFP localized primarily to the oocytes in early adulthood, as previously reported -Expr520, and accumulated in the pseudocoelomic fluid and gonads after reproduction ended. |
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