WormMine: ExpressionPattern Expr54

• Pattern: Staining behind terminal bulb of pharynx in adults, believed to be the lobes of the excretory gland cell. L1 larvae appear to have nuclear localised staining of the intestine cells. There are multiple components to the b-galactosidase expression pattern driven by pUL#161G10. The most striking component is in the excretory gland cell, from late embryogenesis onwards. b-galactosidase appears to fill the two large lobes of this bi-nucleated cell which is formed from the fusion of two cells during development. Occasionally the b-galactosidase appears to be in discrete subcellular compartments restricted to the posterior half of the lobes of the excretory gland. There is also expression in the ventral nerve cord from late embryogenesis onwards and in the intestine in the late embryo and early larval stages.
• Primary Identifier: Expr54
• Remark: Reporter fusion junction is at position 24742 in K05B2 ..CATAGAGAGATTTCAGAATCGGACGGACTGCCGTTTCATCGGTCACTTATTATGAACTTACTTACTGTGG CCCACTAGTCAGTGCACTTGCAACAATATGAACAGCGTTATGTAGTTATGCGGGAGGATTGATCTCTGGAAT AGCATTTATGATTGCACCAGCATCTCCAAATATTTATGTATTTATGTTGATTTACGGAGTGATGGGAGGTCT TGGATTTGGTATGATC/lacZ fusion junction is at position 24742 in K05B2..CATAGAGAGATTTCAGAATCGGACGGACTGCCGTTTCATCGGTCACTTATTATGAACTTACTTACTGTGGCCCACTAGTCAGTGCACTTGCAACAATATGAACAGCGTTATGTAGTTATGCGGGAGGATTGATCTCTGGAATAGCATTTATGATTGCACCAGCATCTCCAATATTTATGTATTTATGTTGATTTACGGAGTGATGGGAGGTCTTGGATTTGGTATGATC/lacZ. Other authors: "Arnold JM", "Herbert R" "Hope IA"Date 1993-01 updated information 01/98

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
neuron with cell body associated with the ventral nerve cord.	ventral cord neuron	ventral cord motoneuron	WBbt:0005300
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
gland cell of the secretory-excretory system, sends processes to ring, opens into excretory duct.	excretory gland cell	exc gl	WBbt:0005776

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003986	mct-4	K05B2.5	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003