WormMine: ExpressionPattern Expr98

• Pattern: A few nuclei stain in the 3-fold embryos, these are probably pharyngeal and/or hypodermal. In L1 larvae we can see expression in the hypodermal nuclei of the head and body, and in the nuclei of the pharyngeal muscles. Staining of the pharyngeal muscle nuclei and hypodermal nuclei is very strong in some L2 larvae and older worms. The varying numbers of nuclei staining may be due to mosaicism. Expression in this multi-component pattern is first seen in late embryogenesis and extends into adulthood. From L2 larvae through to adulthood, we can see expression in the large intestinal nuclei, this sometimes extends to non-localised staining of the intestine. The final component is exhibited in late larvae and adults. Staining is seen in the nucleus of the excretory cell and in the excretory canals extending anteriorly and posteriorly.
• Primary Identifier: Expr98
• Remark: Other strains injected with this plasmid are UL248, UL249, UL250, UL251 and UL252. The expression in these strains are consistant with that shown in UL247 though some appear less extensive.

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
type of cells that make up muscle layers in the pharynx.	pharyngeal muscle cell		WBbt:0005451
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
H-shaped cell associated with the excretory system, largest cell in C. elegans.	excretory cell	excretory canal cell	WBbt:0005812

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004421	rpl-10L	F10B5.1	Caenorhabditis elegans

7 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020