WormMine: ExpressionPattern Expr973

• Pattern: mab-9 is expressed in very few cells throughout development. In L1 larvae, mab-9::GFP expression is seen in the posterior hindgut cells B and F, the two cells that take on inappropriate lineage fates in mab-9 mutants. Expression is also seen in two posterior hypodermal nuclei of the large syncytial cell hyp7 and in the nervous system, where mab-9::GFP expression is evident in two neuronal nuclei of the lumbar ganglion. These are most likely to be the two nuclei of the interneuron PVC. In addition, mab-9::GFP expression is always observed in a single neuronal nucleus of the head ganglion. The neuronal and hypodermal expression pattern of mab-9 is the same in males and hermaphrodites. mab-9::GFP expression is observed in both B.a and B.p. In late larval males, the expression pattern of mab-9 is more widespread in nuclei of the proctodeum. In adult hermaphrodites, mab-9::GFP expression is maintained in the same six cells that expressed mab-9 during larval development. A construct in which a 5.2-kb mab-9 promoter-only cassette was cloned in front of GFP (vector pPD96.04) was found to have a very similar expression pattern when present in wild-type worms as an extrachromosomal array. mab-9 is also expressed during embryogenesis. mab-9::GFP expression is first seen at the 1.5-fold stage, 430 min after first cleavage, in three to four nuclei around the presumptive rectum [B, F, and hyp7]. In 3-fold embryos, mab-9::GFP expression becomes very intense in the nuclei of B and F, and weaker expression can also be observed in nuclei of the ventral cord. This ventral cord expression appears to be transient and is not observed reproducibly as larval development ensues.
• Primary Identifier: Expr973
• Subcellular Localization: mab-9 localizes to the nucleus.

10 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring.	ventral nerve cord	ventral cord	WBbt:0005829
entire syncytium of hyp7	hyp7 syncytium	hyp7	WBbt:0005734
Ventral cord interneuron, cell body in lumbar ganglion, synapses onto VB and DB motor neurons, formerly called delta.	PVCR	lineage name: ABprpppaapaa	WBbt:0004092
Ventral cord interneuron, cell body in lumbar ganglion, synapses onto VB and DB motor neurons, formerly called delta.	PVCL	lineage name: ABplpppaapaa	WBbt:0004094
ganglion in the head.	head ganglion		WBbt:0005135
Rectal cell, postembryonic blast cell in male	B cell	lineage name: ABprppppapa	WBbt:0003825
Rectal cell, blast cell in male	F cell	lineage name: ABplppppapp	WBbt:0004799
cell, posterior daughter of B cell in male	B.p		WBbt:0006986
cell, anterior daughter of B cell in male	B.a		WBbt:0006985
Essensially a modified rectum, junction between the alimentary and genital tracts in the male.	proctodeum		WBbt:0006795

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003106	mab-9	T27A1.6	Caenorhabditis elegans

4 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018
	A developemental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until death.		postembryonic Ce	WBls:0000022