WormMine: ExpressionPattern Expr9595

• Pattern: Strong NPHP-8 expression was found in a subset of ciliated sensory neurons in hermaphrodites. The expression of nphp-8 overlapped with but was not limited to, DiI loaded neurons, including six neurons in the head (ASI, ASH, AWB, ASJ, ASK, and ADL) and two neurons in the tail (PHA and PHB).To determine the subcellular localization of NPHP-8 in C. elegans, an expression construct containing NPHP-8 fused to red fluorescent protein mCherry, driven by its own promoter, was created. The mCherry-tagged NPHP-8 was only observed at the distal end of dendrites of amphid and phasmid neurons in hermaphrodites. The NPHP-8 protein specifically co-localized with NPHP-4 at the transition zones at the bases of the cilia. Little or no fluorescence was found in the ciliary axonemes.
• Primary Identifier: Expr9595
• Reporter Gene: [NPHP-8::SL2-GFP], [NPHP-8::mCherry], translational fusion. Two nphp-8 gene fragments were obtained using PCR. A 5.9 kb fragment containing the 3.9 kb promoter and the genomic sequence (exons and introns) up to exon 6 and an- other 3.0 kb fragment containing all of the exons downstream of exon 6 were amplified from the total cDNA library. These two sequences were inserted into vector pXTL115 in the proper order to generate the translational fusion pXTL123 (NPHP-8::mCherry). To create the SL2 expression vector pXTL162 (NPHP-8::SL2-GFP), the two fragments (the 3.0 kb nphp-8 fragment with a stop codon) were cloned into the pJG7 vector.
• Subcellular Localization: The mCherry-tagged NPHP-8 was only observed at the distal end of dendrites of amphid and phasmid neurons in hermaphrodites. The NPHP-8 protein specifically co-localized with NPHP-4 at the transition zones at the bases of the cilia. Little or no fluorescence was found in the ciliary axonemes.

17 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Amphid wing cells, neurons having ciliated sheet-like sensory endings closely associated with amphid sheath	AWBL	lineage name: ABalpppppap	WBbt:0003829
Amphid wing cells, neurons having ciliated sheet-like sensory endings closely associated with amphid sheath	AWBR	lineage name: ABpraaappap	WBbt:0003828
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASKL	lineage name: ABalpppapppa	WBbt:0003884
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASKR	lineage name: ABpraaaapppa	WBbt:0003883
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASHL	lineage name: ABplpaappaa	WBbt:0003890
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASHR	lineage name: ABprpaappaa	WBbt:0003889
one member of amphid neurons, single ciliated ending, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASIL	lineage name: ABplaapapppa	WBbt:0003888
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASIR	lineage name: ABpraapapppa	WBbt:0003887
Amphid neuron, prob. chemosensory	ADLL	lineage name: ABalppppaad	WBbt:0003997
Amphid neuron, prob. chemosensory	ADLR	lineage name: ABpraaapaad	WBbt:0003995
Phasmid neurons, probably chemosensory	PHAL	lineage name: ABplpppaapp	WBbt:0004366
Phasmid neurons, probably chemosensory	PHAR	lineage name: ABprpppaapp	WBbt:0004365
Phasmid neurons, probably chemosensory	PHBL	lineage name: ABplapppappp	WBbt:0004364
Phasmid neuron, probably chemosensory	PHBR	lineage name: ABprapppappp	WBbt:0005659
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASJR	lineage name: ABpraaappppa	WBbt:0003885
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASJL	lineage name: ABalpppppppa	WBbt:0003886
neuron type, neurons that have ciliated nerve endings.	ciliated neuron		WBbt:0006816

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00007490	mks-5	C09G5.8	Caenorhabditis elegans

0 Life Stages