WormMine: ExpressionPattern Expr3826

• Pattern: The resulting kel-8::gfp transgene was introduced into nematodes, where it expressed KEL-8::GFP protein in a subset of neurons. KEL-8::GFP and GLR-1::RFP were expressed in the same subset of interneurons, including AVA, AVB, AVD, and AVE as well as PVC.
• Primary Identifier: Expr3826
• Remark: Authors also generated a transgene, Pglr-1::kel-8::yfp, that expresses (via the glr-1 promoter) KEL-8 protein fused at its carboxy terminus to YFP. Pglr-1::kel-8::yfp was introduced as an extra-chromosomal transgene into kel-8 mutant nematodes that express an integrated glr-1::cfp transgene. Although kel-8 mutants accumulate large accretions of GLR-1::CFP in the absence of exogenous KEL-8 protein, sibling kel-8 mutants that express KEL-8::YFP from a Pglr-1::kel-8::yfp transgene have properly-localized GLR-1::CFP (4 independent lines, 15 - 20 animals examined per line). These results indicate that the KEL-8 fusion protein is functional.
• Subcellular Localization: KEL-8::GFP was localized to clusters along the ventral cord neurites. Coexpression of KEL-8::GFP and GLR-1::RFP resulted in KEL-8::GFP clusters that were localized adjacent to GLR-1::RFP clusters.

10 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Ventral cord interneuron, cell body in lumbar ganglion, synapses onto VB and DB motor neurons, formerly called delta.	PVCR	lineage name: ABprpppaapaa	WBbt:0004092
Ventral cord interneuron, cell body in lumbar ganglion, synapses onto VB and DB motor neurons, formerly called delta.	PVCL	lineage name: ABplpppaapaa	WBbt:0004094
Ventral cord interneuron, synapses onto VA, DA, and AS motor neurons; formerly called alpha	AVAL	lineage name: ABalppaaapa	WBbt:0003870
Ventral cord interneuron, synapses onto VA, DA, and AS motor neurons; formerly called alpha	AVAR	lineage name: ABalaappapa	WBbt:0003869
Ventral cord interneuron, synapses onto VB and DB motor neurons; formerly called beta.	AVBL	lineage name: ABplpaapaap	WBbt:0003868
Ventral cord interneuron, synapses onto VB and DB motor neurons; formerly called beta.	AVBR	lineage name: ABprpaapaap	WBbt:0003867
Ventral cord interneuron, like AVD but outputs restricted to anterior cord.	AVEL	lineage name: ABalpppaaaa	WBbt:0003864
Ventral cord interneuron, like AVD but outputs restricted to anterior cord.	AVER	lineage name: ABpraaaaaaa	WBbt:0003863
Ventral cord interneuron, synapses onto VA, DA, AS motorneurons; formerly called delta.	AVDL	lineage name: ABalaaapalr	WBbt:0003866
Ventral cord interneuron, synapses onto VA, DA, AS motorneurons; formerly called delta.	AVDR	lineage name: ABalaaapprl	WBbt:0003865

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00020952	kel-8	W02G9.2	Caenorhabditis elegans

0 Life Stages