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Expr4381
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Expressed in AVA, AVB, DVA, I5, RID, RIM, PVQ, SAA, SIA, SIB, SMB(?), SMD, ventral cord motor neurons, some unidentified neurons in the head. Also expressed head muscles (weak), body wall muscles (weak). |
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Picture: N.A. |
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Marker92
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Marker for AVB neurons. |
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Expr15558
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Expr15567
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Expr15571
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Expr15572
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Expr15573
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Expr15579
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Expr15586
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Expr15651
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Expr15652
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Expr15589
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Expr15591
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Expr12196
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To determine where lite-1 is expressed and identify likely sites of lite-1 function, the authors generated and examined worms carrying one of four transgenes derived from the wild-type lite-1 locus: a genomic fragment (lite-1 genomic), a transcriptional fusion (lite-1prom::gfp), a C-terminal translational fusion (lite-1prom::lite-1::gfp), and an N-terminal translational fusion (lite-1prom::gfp::lite-1). GFP expression was observed in a total of 29 cells: pharyngeal neurons M1, M4, M5, and MI; non-pharyngeal neurons ASK, ADL, ASI, ASH, AVG, AVB, RIM, ADF, PHA, PHB, and PVT; and non-neuronal cells Hyp3 (hypoderm), AMso (amphid socket cells), and PHso (phasmid socket cells). AVB was observed only with the C-terminal fusion transgene, and RIM and ADF were observed only with the transcriptional fusion transgene. lite-1 expression in AVG and PVT was previously reported. |
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Expr15598
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Picture: Fig 3. |
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Expr8850
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Neuronal Expression: AVA, AVB, AVE, PVC, AIB, AUA, AVG, RIB, RIC, SAA, SIA, SIB, RIF, RIM, RMD, RME, SMD, DA, DB, VA, VB, M5, NSM, MC, I3, MI?. Non-neuronal Expression: rectal epithelium, body wall muscle, spermethecae, vulva muscle. |
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Expr15604
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Expr14590
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Embryonic expression of exc-7 was first observed at the bean stage. By reverse lineaging with use of SIMI-Biocell software, we confirm the identity of one of the expressing cells at this stage as the excretory canal cell. In L1 animals, broad expression in the head, ventral nerve cord (VNC), and tail was observed. In young adults, expression is notably observed in vulva cells. In the nervous system specifically, expression is observed in many neurons throughout the body, but unlike Drosophila Elav, exc-7::gfp it is not panneuronally expressed. We confirmed previously reported expression in cholinergic VNC MNs, but absence of GABAergic VNC MNs, consistent with previous reports (Fujita et al., 1999; Loria et al., 2003) and consistent with exc-7 functioning in cholinergic, but not GABAergic neurons to control alternative splicing (Norris et al., 2014). exc-7::gfp is also expressed in some non-neuronal cell types, including muscle and hypodermis, but not in the gut. A previous report showed that exc-7 is only transiently and weakly expressed in the excretory cell, which, based on exc-7's excretory mutant phenotype, has puzzled researchers (Fujita et al., 2003). We find that the gfp tagged exc-7 locus is strongly and continuously expressed in the excretory canal cell. |
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Expr15608
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Expr15611
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Expr1500
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Both: The transgene constructs showed minimal expression in non-neuronal cells. However, transgene expression in intestinal, hypodermal, or muscle cells was observed in occasional transgenic lines in which 3.0-kb genomic DNA fragments extending just past the CePPEF start codon directed production of short N-terminal regions of CePPEF fused to GFP. As these expression patterns occurred sporadically, they likely reflect transgene effects rather than an expression pattern relevant to the endogenous CePPEF gene. FL-GFP: FL-GFP transgene directed GFP expression to the same set of neurons as the NLS-GFP-LacZ construct, and expression in a single posterior neuron was also more clearly observed. Within the expressing cells, the FL-GFP fusion protein localized efficiently to several structures beyond the cell soma, including axons that form the ventral nerve cord; dendrites that extend to the anterior tip of the animal; and the cilia of neurons AWB, AWC, and BAG. aa-(1)-NLS-GFP-LacZ: Injection of the aa-(1)-NLS-GFP-LacZ construct into C. elegans revealed reporter gene expression in several anterior neurons, including AWB, AWC, AVA, AVB, AVX, BAG, and URX. The ASE neuron showed inconsistent transgene expression. |
FL-GFP: Within the expressing cells, the FL-GFP fusion protein localized efficiently to several structures beyond the cell soma, including axons that form the ventral nerve cord; dendrites that extend to the anterior tip of the animal; and the cilia of neurons AWB, AWC, and BAG. aa-(1)-NLS-GFP-LacZ: GFP-LacZ fusion protein facilitates retention in the nucleus. |
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Picture: Fig 4A to D. |
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Expr8613
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lgc-55::mCherry and lgc-55::GFP transgenic animals showed reporter expression in a subset of neck muscles and a restricted set of neurons.These neurons aare AVB, RMD, SMDD, SMDV, IL1D, IL1V, SDQ, HSN, and ALN neurons. In addition, weak lgc-55 reporter expression was also detected in the UV1 cells and tail muscle cells. |
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This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
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Expr716
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Expression observed in most or all neurons at high levels as well as in some hypodermal and muscle cells. In most neurons, sax-3::GFP expression is transient, peaking during the period of axon outgrowth. Highest level of expression is observed in the embryo, particularly during the initial period of axon outgrowth at 350-400 min (comma-stage). At comma-stage high level of expression is observed in anterior embryo, including most developing neurons of the nerve ring and a swath of ventral cells that includes the developing motor neurons of the ventral nerve cord and posterior neurons such as PVQ. Lower level of expression is observed in the epidermal cells. 200-400 - cell stage; 200-300 min of development: Expression seen in all epidermal cells at low level. 3-fold stage; >500 min of development: Expression seen in muscles that extend from nerve ring to the anterior tip of the head. L1: Expression in motor neurons in the head including RMD, RMG, SMD, SIA and SIB neurons; projection interneurons in the head and tail, including AVA, AVB, PVC, AVD, PVQ and ALA neurons; and the sensory OLQ neuron. This neuronal expression diminishes throughout postembryonic development. During L1, expression persists in the head muscles and appears in muscles along the body wall, with ventral muscles expressing more strongly than posterior muscles. This expression continues until the adult stage. Epidermal expression was rarely seen in larval stages. L2: Expression observed in HSN motor neurons. HSN motor neurons extends a growth cone (axon from their lateral cell bodies) towards the ventral midline and expression is at its highest at this point. L3: Expression observed in the axon reaching the ventral nerve cord. L4: Expression continues in HSN, when HSN axon grows anteriorly to the head. Adult: Expression decreases in HSN at this stage after the completion of HSN axon outgrowth. In addition expression observed in motor neurons, interneurons and sensory neurons listed above also postembryonic ventral cord motorneurons, some interneurons from the tail and head, body wall and vulval muscles. |
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Expr15402
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Expr15406
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Expr15410
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sra-11(pB) |
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Expr12748
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RAB-6.1 is broadly expressed in most tissues, with a large degree of overlapping expression with RAB-6.2. RAB-6.1::GFP is highly expressed in body wall muscle, intestine, somatic gonad, distal tip cells, vulva, and neurons. RAB-6.1 is expressed in multiple GLR-1-expressing neurons, including AVB, AVD, RIG, and PVC, but not AVA or RMDV. |
RAB-6.1 colocalizes with RAB-6.2 at the Golgi in neurons. RAB-6.1 is localized to intestinal Golgi. |
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Picture: Fig 3. |
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Expr8844
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Neuronal Expression: ASI, ASJ, AVB, AVE, PVC, AIB, AIN, DA, DB, M2, NSM. Non-neuronal Expression: pharynx, GLR cells. |
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Picture: Figure 7. |
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Expr7808
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A highly reproducible pattern of DKF-1 expression was observed as animals matured from embryo to adult. Intense fluorescence signals corresponding to DKF-1GFP revealed robust kinase accumulation in both (a) a region bounded by the anterior and posterior bulbs of the pharynx and (b) a tail area that contains lumbar, dorsorectal and pre-anal ganglia. Specifically, DKF-1 is differentially enriched in a cluster of cells that are immediately adjacent to the posterior pharyngeal bulb. Strong signals also emanate from cells positioned along the lateral surface of this bulb in animals carrying the dkf-1P::DKF-1GFP transgene. At the anterior pharyngeal bulb, DKF-1 accumulates selectively in bodies and in very thin processes (dendrites and axons) of two neurons. Nearly all cells expressing DKF-1 appear to be neurons. Two fluorescent cells with similar sizes and locations (at the anterior edge of the isthmusposterior bulb) may be M2 motor neurons. The location of the more posterior fluorescent neuron approximates the position of the cell body of an NSM neuron. DKF-1 also accumulates in a cell resembling I1. Other candidate DKF-1-enriched cells in the pharyngeal region include: the AWB, ADL, and ADF chemosensory neurons; and AVB and AIA interneurons.n C. elegans tail, DKF-1GFP expression is differentially elevated in neurons located within the dense neuropile of several tail ganglia. The pattern of fluorescence reveals that cell bodies and/or processes of phasmid neurons (PHA, PHB, and PHC), interneurons (PVC, DVA, DVB, PVQ, PVT) and motor neurons (VD13, DD6, VA12) are candidate sites for accumulation of DKF-1 protein. |
Expressed in neuronal cell bodies and/or processes. |
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Since these gfp fusions lack the introns and the 3' untranslated region, they might be lacking potential regulatory sequences. In that case, the gfp expression patterns may not precisely represent those of the endogenous kin-8 gene. cam-1 is called kin-8 in this article. |
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Expr2267
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Expressed in chemosensory neurons in amphid: ASH. Other sensory neurons: ADE, FLP. Touch receptor neurons: AVM, ALM, PVM, PLM. Amphid interneurons: AIY, AIZ. Other interneurons: RIC, RMG, RIS, DVA, AVA, AVE, PVC, AVK, PVQ. Interneurons?: ALN, BDU, SDQ. Ring motor/inter neurons: RMD, RMDV. Ring motor neurons: RMED, RMEV Five neurons out of the following six, RIV, AVH, AVB, AVJ, AVD, AIN. About seven neurons in retrovesicular ganglion. Pharyngeal muscles in procorpus and isthmus. M4 and several pharyngeal neurons. A part of intestine and a few body wall muscles near the head (weak). Distal tip cells (sometimes and weak). A few ventral motor neurons and seam cells (rarely and weak). The expression patterns did not appear to change through the larva to adult stages. Embryonic expression was also observed. |
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