WormMine: ExpressionPattern Expr12270

• Pattern: Transgenic embryos carrying a 3x28 bp tandem repeat in an otherwise promoter-less GFP reporter, showed expression in a pair of head neurons with dendrites extending to the tip of the head, consistent with ASJ morphology and position. However, once the embryo hatched, the number of animals with GFP-fluorescent ASJ neurons decreased dramatically. Very few L1 larvae showed expression in ASJ, but none in L2-L4 larvae or adult animals. This is most likely due to the requirement of additional cis-regulatory regions in the surrounding promoter area necessary for post-embryonic maintenance of trx-1 expression. A bipartite motif, within the trx-1 promoter, composed of two 6 bp elements (CAACCC and AATTAA), separated by a 3 bp linker, is absolutely required for ASJ expression. Hence, mutation of either of the two individual 6 bp elements resulted in the abolishment of GFP expression in ASJ neurons, while mutation of the 3 bp linker did not disrupt GFP expression.
• Primary Identifier: Expr12270

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASJR	lineage name: ABpraaappppa	WBbt:0003885
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASJL	lineage name: ABalpppppppa	WBbt:0003886

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00015062	trx-1	B0228.5	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003