WormMine: ExpressionPattern Expr2562

• Pattern: Another notable aspect of ceh-13 expression in ray complexes 5, 7, and 9 was its cell-specific regulation. At the beginning of L4, the highest levels of protein were present in A- and B-type neurons, intermediate levels in the support cells, and lowest levels in hypodermal cells. Protein levels were higher in hyp7 than in hyp5,9. Upon tail retraction, expression in hyp7 was transiently upregulated and maintained high until mid-L4. At this point, there was upregulation of expression in st5, 7, and 9 and downregulation in all neuroblasts, as well as in hyp7. During tail morphogenesis in late L4 and in the course of ray shaping, significant upregulation of cytoplasmic CEH-13::GFP reporter levels occurred in structural cells, particularly around the papillae. In tests for ceh-13 expression in the sensory rays, ray-specific CEH-13 and CEH-13::GFP expression was detected shortly after the L3 moult, concomitantly with completion of the last divisions of the precursor cells. The protein initially exhibited uniform cytoplasmic distribution, then rapidly accumulated in the nuclei. On the basis of the complex patterning and dynamics of expression, two aspects of activity could be defined. In the first, ceh-13 was expressed in the A-type neurons of all rays, although lower levels were seen in the ventral ray groups 2, 4, and 8. This expression persisted until mid-L4 and was switched off thereafter except in ray 9. Thus, with the exception of R9A, detectable levels of protein were no longer present in the A-type neurons by the time of ray morphogenesis and the emergence of papillae. In the second aspect, CEH-13 and CEH-13::GFP were specifically expressed in the dorsal ray groups 5, 7, and 9, where they were present in all cells after completion of the last division of the precursor cells. Detectable levels of protein rapidly disappeared from the hypodermal cells in groups 5 and 9 but persisted in the hypodermal cell of 7 until the beginning of tail retraction. Three other ceh-13-expressing cells, R(5,7,9).aap, underwent apoptotic cell death shortly after division of the anterior neuroblasts that generated them. Thus, at the beginning of tail retraction, remaining ceh-13 activity could be observed exclusively in ray groups 5, 7, and 9, i.e., in B-type neurons of 5 and 7, in the A-type neuron of 9, and in the structural cells of 5, 7, and 9. The first expression of CEH-13 in the male tail was detected during the third larval stage (L3) in B_gamma. Upon division of the B_gamma cell, the protein became equally distributed between the anterior and posterior daughters B_gamma.a and B_gamma.p, but subsequently disappeared in B_gamma.p. CEH-13 and CEH-13::GFP expression in B_gamma.a was accompanied by subcellular redistribution of the protein, with an increase in nuclear and a decrease in cytoplasmic accumulation. After division of B_gamma.a, CEH-13 exhibited exclusive nuclear localisation in the two daughters B_gamma.al and B_gamma.ar. Also during L3, sex independent expression of CEH-13 in the tail region could be observed in an asymmetric neuron tentatively identified as Ql.ap. In the L4, several additional unidentified expressing cells were observed.
• Primary Identifier: Expr2562
• Subcellular Localization: Expression was both cytoplasmic and nuclear, and the nuclear expression appeared stronger in the region of the nucleolus.

35 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Neuron class of one sensory neuron, basal body, not part of a sensillum, projects into preanal gangion.	PQR	lineage name: QL.ap	WBbt:0004096
Post-embryonic cell of pedigree V6L.pppapaaa, male sensory rays, neuron, striated rootlet.	R4AL	lineage name: V6L.pppapaaa	WBbt:0004030
Post-embryonic cell of pedigree V6R.pppapaaa, male sensory rays, neuron, striated rootlet.	R4AR	lineage name: V6R.pppapaaa	WBbt:0004029
Post-embryonic cell of pedigree V6L.papapaaa, male sensory rays, neuron, striated rootlet.	R2AL	lineage name: R2L.aaa	WBbt:0004042
Post-embryonic cell of pedigree V6R.papapaaa, male sensory rays, neuron, striated rootlet.	R2AR	lineage name: R2R.aaa	WBbt:0004041
Post-embryonic cell of pedigree TL.apappapa, male sensory rays, neuron, darkly staining tip, open to outside.	R7BL	lineage name: TL.apappapa	WBbt:0004001
Male sensory rays, neuron, darkly staining tip, open to outside	R7BR	lineage name: TR.apappapa	WBbt:0004000
Post-embryonic cell of pedigree V6L.ppppaapa, male sensory rays, neuron, darkly staining tip, open to outside.	R5BL	lineage name: V6L.ppppaapa	WBbt:0004022
Post-embryonic cell of pedigree V6R.ppppaapa, male sensory rays, neuron, darkly staining tip, open to outside.	R5BR	lineage name: V6R.ppppaapa	WBbt:0004021
Male sensory rays, structural cell	R9stR	lineage name: TR.appapapp	WBbt:0003962
Post-embryonic cell of pedigree TL.apappapp, male sensory rays, structural cell.	R7stL	lineage name: TL.apappapp	WBbt:0003998
Male sensory rays, structural cell	R7stR	lineage name: TR.apappapp	WBbt:0003996
Post-embryonic cell of pedigree TL.appapapp, male sensory rays, structural cell.	R9stL	lineage name: TL.appapapp	WBbt:0003964
Post-embryonic cell of pedigree V6L.ppppaapp, male sensory rays, structural cell.	R5stL	lineage name: V6L.ppppaapp	WBbt:0004020
Post-embryonic cell of pedigree V6R.ppppaapp, male sensory rays, structural cell.	R5stR	lineage name: V6R.ppppaapp	WBbt:0004019
Post-embryonic cell of pedigree V6L.pppppaaa, male sensory rays, neuron, striated rootlet.	R6AL	lineage name: V6L.pppppaaa	WBbt:0004018
Post-embryonic cell of pedigree V6R.pppppaaa, male sensory rays, neuron, striated rootlet.	R6AR	lineage name: V6R.pppppaaa	WBbt:0004016
Post-embryonic cell of pedigree V5L.pppppaaa, male sensory rays, neuron, striated rootlet.	R1AL	lineage name: R1L.aaa	WBbt:0004052
Post-embryonic cell of pedigree V5R.pppppaaa, male sensory rays, neuron, striated rootlet.	R1AR	lineage name: R1R.aaa	WBbt:0004050
Male sensory rays, neuron, striated rootlet	R9AR	lineage name: TR.appapaaa	WBbt:0003970
Male sensory rays, neuron, striated rootlet	R7AR	lineage name: TR.apappaaa	WBbt:0004002
Post-embryonic cell of pedigree V6R.ppppaaaa, male sensory rays, neuron, striated rootlet.	R5AR	lineage name: V6R.ppppaaaa	WBbt:0004023
Male sensory rays, neuron, striated rootlet	R9AL	lineage name: TL.appapaaa	WBbt:0003971
Post-embryonic cell of pedigree TL.apappaaa, male sensory rays, neuron, striated rootlet.	R7AL	lineage name: TL.apappaaa	WBbt:0004006
Post-embryonic cell of pedigree V6R.papppaaa, male sensory rays, neuron, striated rootlet.	R3AR	lineage name: V6R.papppaaa	WBbt:0004035
Post-embryonic cell of pedigree TL.appaaaaa, male sensory rays, neuron, striated rootlet.	R8AL	lineage name: TL.appaaaaa	WBbt:0003994
Male sensory rays, neuron, striated rootlet	R8AR	lineage name: TR.appaaaaa	WBbt:0003992
Post-embryonic cell of pedigree V6L.ppppaaaa, male sensory rays, neuron, striated rootlet.	R5AL	R5L.aaa	WBbt:0004024
Post-embryonic cell of pedigree V6L.papppaaa, male sensory rays, neuron, striated rootlet.	R3AL	lineage name: R3L.aaa	WBbt:0004036
Gonochoristic organism that can produce male gametes.	male	male organism	WBbt:0007850

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000437	ceh-13	R13A5.5	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038