WormMine: ExpressionPattern Expr11400

• Pattern: NLP-22 is selectively expressed, in a single-neuron pair, the RIAs. NLP-22 is predicted to be secreted. When GFP was fused to the C-terminus of the NLP-22 preproprotein, green fluorescence was observed in several neurons in the head as well as in the ventral nerve cord, supporting the notion that NLP-22 is a secreted peptide. mRNA abundance of the gene nlp-22 was higher in the L4 stage than in lethargus. Using qRT-PCR, we confirmed that nlp-22 mRNA expression cycles with a constant phase relationship to lethargus. nlp-22 mRNA peaked midway through the active larval stages, decreased during each lethargus stage, and was absent in adults.
• Primary Identifier: Expr11400
• Reporter Gene: [pnlp-22::GFP] transcriptional fusion and [Pnlp-22::nlp-22:gfp; Pmyo-2::mCherry; unc-119(+)] translational fusion. The transcriptional reporter for nlp-22 (Pnlp-22:Intron:gfp) was made by fusing the promoter of nlp-22 (-450 to +3) to the coding sequence of GFP, amplified from pPD95.75 and replacing one of the synthetic introns of gfp with the intron of nlp-22( +85 to +481).

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring.	ventral nerve cord	ventral cord	WBbt:0005829
anterior-most body region containing the pharynx.	head		WBbt:0005739
Ring interneuron, many synapses	RIAL	lineage name: ABalapaapaa	WBbt:0003960
Ring interneuron, many synapses	RIAR	lineage name: ABalaapppaa	WBbt:0003958

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003760	nlp-22	T24D8.3	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023