WormMine: ExpressionPattern Expr8117

• Pattern: It is highly and ubiquitously expressed in embryonic cells, although expression was not detected prior to the 30-cell stage, and often appears stronger in the anterior of the embryos. Expression is also observed during the first larval (L1) stage in the nuclei of ventral cord neurons, and in intestinal nuclei, which undergo endoreduplication before each molt. Consistent with the timing of intestinal endoreduplication cycles, UNC-85-GFP is expressed in the intestinal nuclei at later larval stages prior to each molt. From the mid-third (L3) through the fourth (L4) larval stages, expression is observed in vulval precursor cells, which divide to generate the vulval primordial cells. GFP expression is not detected in the germline at any stage of development, or in early embryos, most likely due to germline-specific silencing of transgenes from high-copy arrays. GFP expression was also not observed in adult hermaphrodites, which have completed all of their cell lineages.
• Primary Identifier: Expr8117
• Remark: Picture: Figure 3.
• Subcellular Localization: UNC-85-GFP is localized to nuclei.

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a cellular object that consists of subcellular components, expresses genes or functions.	Cell	Cell type	WBbt:0004017

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006817	unc-85	F10G7.3	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003