| Primary Identifier | Expr8133 | Remark | Picture: Figure 7, Figure S2. |
| Subcellular Localization | CAR-1 is an Scd6 homolog that reside in a variety of RNA regulatory particles, including P bodies, P granules, Drosophila neuronal granules, and mammalian stress granules. In C. elegans, primarily two types of CAR-1 foci exist: those with P body proteins, such as DCAP-1, and those with P granule protein PGL-1. Significant overlap of LARP-1 and CAR-1 were observed in the mid-pachytene region. Since LARP-1 is excluded from P granules (see Expr8132, authors reasoned that the LARP-1/CAR-1 foci were P bodies. Moreover, as with DCAP-1 and LARP-1 colocalization, only a subset of CAR-1 and LARP-1 foci colocalized. These results confirm that LARP-1 accumulates in a subset of germline P bodies. Wild-type and larp-1(0) germ lines were costained with aLARP-1 and a polyclonal antibody directed against DCAP-1. DCAP-1 was predominately cytoplasmic and accumulated in irregularly shaped puncta. DCAP-1 was most abundant in the mitotic region, mid- pachytene region, and distal oocytes and least abundant in the transition zone, early pachytene region, and most proximal oocytes. LARP-1 and DCAP-1 overlapped throughout the distal germ line and colocalized in perinuclear foc. However, not all DCAP-1 foci stained positively for aLARP-1 and not all LARP-1 foci were positive for aDCAP-1. These results indicate that LARP-1/DCAP-1 foci define a unique subset of P bodies in the germ line. |
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes. | germ line | germline | WBbt:0005784 |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00020097 | larp-1 | R144.7 | Caenorhabditis elegans |
