WormMine: ExpressionPattern Expr2897

• Pattern: The hlh-14::gfp transgene expresses GFP in the nuclei of several cells in the developing embryo. Prior to morphogenesis, a horseshoe-shaped pattern of cells expresses HLH-14::GFP in the anterior embryo. The expressing cells appear to be neuroblasts but have not been positively identified. During morphogenesis, HLH-14::GFP assumes a complicated, diverse pattern, and no HLH-14::GFP is visible during postembryonic development. In the posterior embryo, HLH-14::GFP was first detect in the bilaterally symmetric blast cells ABplapppa and ABprapppa. A centrally located posterior blast cell, identified as C.aapa, expresses HLH-14::GFP a little later. Around 230 minutes of development, each PVQ/HSN/PHB neuroblast divides to generate an anterior PVQ neuroblast and a posterior HSN/PHB neuroblast. HLH-14::GFP was seen expressed in both of these daughter cells. At 280 minutes of development, the HSN/PHB neuroblast divides to produce a small daughter cell that dies and a larger daughter cell, the HSN/PHB precursor. Both cells contain HLH-14::GFP. Finally, around 310 minutes of development, the PVQ neuroblast divides to produce a posterior daughter cell that dies and the PVQ neuron. Again, HLH-14::GFP was seen in both of these cells.
• Primary Identifier: Expr2897
• Subcellular Localization: Expressed in nuclei.

19 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNL	lineage name: ABplapppappa	WBbt:0004758
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNR	lineage name: ABprapppappa	WBbt:0004757
Interneuron, projects along ventral cord to ring	PVQL	lineage name: ABplapppaaa	WBbt:0004076
Interneuron, projects along ventral cord to ring	PVQR	lineage name: ABprapppaaa	WBbt:0004074
Phasmid neurons, probably chemosensory	PHBL	lineage name: ABplapppappp	WBbt:0004364
Phasmid neuron, probably chemosensory	PHBR	lineage name: ABprapppappp	WBbt:0005659
embryonic cell	ABprapppa		WBbt:0005909
embryonic cell	ABprapppaap		WBbt:0005937
embryonic cell	ABplapppaa		WBbt:0005935
embryonic cell	ABplapppapp		WBbt:0005970
embryonic cell	Caapa		WBbt:0006123
embryonic cell	ABprapppap		WBbt:0006144
embryonic cell	ABprapppaa		WBbt:0006234
embryonic cell	ABplapppapa		WBbt:0006208
embryonic cell	ABprapppapa		WBbt:0006361
embryonic cell	ABplapppap		WBbt:0006434
embryonic cell	ABplapppa		WBbt:0006697
embryonic cell	ABplapppaap		WBbt:0006671
embryonic cell	ABprapppapp		WBbt:0006743

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001958	hlh-14	C18A3.8	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 0-350min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 560 cells. From start of first cleavage until cleavage is over.		proliferating embryo Ce	WBls:0000004