WormMine: ExpressionPattern Expr887

• Pattern: Staining wild-type C. elegans with anti-UNC-1 antibody showed a punctate distribution of protein along nerve tracts. Staining was most intense in the nerve ring, the retrovesicular ganglion, and the ventral and dorsal nerve cords. Staining was also prominent in the nerve tracts of the snout, around the vulva, and along the sublateral nerve tracts of the body. No staining was seen in other tissues. Staining was noted in late embryos in the nerve ring and ventral nerve cord.
• Primary Identifier: Expr887
• Remark: Incubation of UNC-1 antibody with fc53, a null allele of unc-1, showed no staining.The staining of N2 was consistent with the fluorescence reported previously in live unc-1(0) animals rescued with a GFP-UNC-1 fusion construct. Antibody staining of the dominant unc-1 allele n494 showed a pattern similar to that for N2; however, the class II allele n774 showed less oveall staining than N2 in a grossly normal distribution. The cellular distribution of UNC-1 was localized by co-staining with DAPI, for nuclear staining, and by co-staining with anti-UNC-17 (vesicular acetylcholine transporter, VAChT), a marker of cholinergic synapses. late embryo(author) = fully-elongated embryo(curator)
• Subcellular Localization: UNC-1 was not found in the nucleus but, rather, was distributed along the presumed axons of neurons.

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring.	ventral nerve cord	ventral cord	WBbt:0005829
the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes.	nerve ring	circumpharyngeal nerve ring	WBbt:0006749
a bundle of nerve processes that runs along the dorsal mid-line of the animal.	dorsal nerve cord	dorsal cord	WBbt:0006750
Ganglion that lies at the anterior limit of the ventral nerve cord, near the ventral ganglion and nerve ring in the head posterior to the excretory pore. It is open and continuous with the region containing the motoneurons of the ventral cord. In the early L1 this ganglion holds 12 neuron cell bodies plus one neuroblast (Sulston and Horvitz, 1977; White et al., 1986). In the adult animal, the ganglion holds 20 neuron cell bodies.	retrovesicular ganglion		WBbt:0005656

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006741	unc-1	K03E6.5	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021