WormMine: ExpressionPattern Expr105

• Pattern: This pattern is first expressed as a few nuclei in 3-fold embryos. A few nuclei also stain in young larvae. In adults expression is evident posteriorly to the vulva. This expression is reproducible but seems to be on the threshhold of observability as sometimes expression is not seen (due to less efficient staining?) It appears to stain the A and B epithelial cells which connect the uterus to the hypodermal syncytium, ie in the hypodermal ridge posterior to the vulva. We have seen one example (due to the low threshhold?) where the posterior third of the anterior component of the A and B epithelial cells, as well as the posterior component stain. This does seem to follow with the possibility of some concentration gradient evident in the gene product produced. There is occasionally a second component. This is seen in the larval stages and is anterior, and more frequently posterior, intestinal nuclei. In adults some diffuse intestinal staining is also seen.
• Primary Identifier: Expr105
• Remark: Other strain-- UL305 late embryo(author) = 3-fold embryo(curator).

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
adult vulval toroid cell, B2.	vulB2		WBbt:0006764
adult vulval toroid cell, B1, formed by fusion of precursors of lineage names P5.papa and P7.ppap.	vulB1		WBbt:0006763
adult vulval toroid cell, A, formed by fusion of precursors of lineage names P5.paaa, P5.paap, P7.pppa and P7.pppp	vulA		WBbt:0006762

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00018408	F44B9.5	F44B9.5	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020