WormMine: ExpressionPattern Expr10566

• Pattern: gfp::nrfl-1 was expressed in the excretory canal, the worm counterpart of the mammalian renal tubules, and the intestine. Although previous large-scale gene expression profiling efforts using nrfl-1-promoter::gfp constructs observed GFP signals in the pharynx, intestine, excretory cells, and some tail cells, the gfp::nrfl-1 construct was exclusively expressed in the intestine and the excretory system. This pattern stayed consistent throughout the larval and adult stages.
• Primary Identifier: Expr10566
• Subcellular Localization: Using a specific antibody raised against the NRFL-1 (C01F6.6a) protein, endogenous NRFL-1 was detected in the luminal side of the intestine. However, the excretory canal was not stained with the antibody. NRFL-1 is found apical to IFB-2 (Intermediate Filament, B family-2) which localizes just beneath the intestinal microvilli, suggesting the enrichment in the intestinal microvilli. Immunofluorescence staining using anti-NRFL-1 antibody detected NRFL-1 on the intestinal luminal membrane.

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Four processes (canals) of the excretory canal cell each contain a central collecting lumen which feeds to a central lumenal canal in the cell body; the central canal forms a specialized membrane to release fluids into the excretory duct. These five canals form a continuous H-shaped channel which extends almost the full length of the body, generally in contact with the lateral hypodermis and the pseudocoelom.	excretory canal	canal	WBbt:0005775

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006438	nrfl-1	C01F6.6	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023