WormMine: ExpressionPattern Expr11335

• Pattern: Multiple copies of either the individual or the paired Cb-ges-1 WGATAR sites, in either orientation and with either C. elegans or C. briggsae as transformation hosts, produce intense reporter gene expression specifically in the embryonic gut. In both host species, reporter expression was first observed at the 4E cell stage of development,the stage at which endogenous ges-1 expression normally can first be detected. Although these reporter constructs initiate expression atthe correct stage, expression is not maintained much laterthan the L2 larval stage in either species;in contrast, endogenous Ce-ges-1 expression continues toincrease throughout the life of the worm. When only a single copy of either the upstreamWGATAR site, the downstream WGATAR site, or thetandem pair of sites was inserted into the test vector andintroduced into C. elegans, no expression in transformedembryos could be detected, either within the gut or elsewhereand even with prolonged staining for b-galactosidaseactivity
• Primary Identifier: Expr11335
• Remark: Feature : "ges-1.WGATAR"

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00028697	Cbr-ges-1	CBG06418	Caenorhabditis briggsae

7 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation.		gastrulating embryo Ce	WBls:0000010
	The C. elegans life stage spanning 20-40min after first cleavage at 20 Centigrade. Contains 4 cells.		4-cell embryo Ce	WBls:0000008